{"_buckets": {"deposit": "5d5e0a65-07c5-4799-ac60-d5bee0f9801e"}, "_deposit": {"created_by": 11, "id": "3629", "owners": [11], "pid": {"revision_id": 0, "type": "depid", "value": "3629"}, "status": "published"}, "_oai": {"id": "oai:shiga-med.repo.nii.ac.jp:00003629", "sets": ["228"]}, "author_link": ["7172", "7245", "8011", "644", "7637", "8009", "8008", "8010"], "item_4_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-06-22", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}, {"bibliographic_title": "International journal of hematology", "bibliographic_titleLang": "en"}]}]}, "item_4_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "藤城, 綾"}], "nameIdentifiers": [{"nameIdentifier": "7245", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "岩佐, 磨佐紀"}], "nameIdentifiers": [{"nameIdentifier": "7172", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "0000-0003-4116-7184", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "https://orcid.org/0000-0003-4116-7184"}]}, {"creatorNames": [{"creatorName": "前川, 平"}], "nameIdentifiers": [{"nameIdentifier": "8009", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "安藤, 朗"}], "nameIdentifiers": [{"nameIdentifier": "644", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90252395", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90252395"}, {"nameIdentifier": "0000-0001-8533-2669", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "https://orcid.org/0000-0001-8533-2669"}]}]}, "item_4_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Vitamin K2 in the form of menatetrenone has clinical benefits for osteoporosis and cytopenia. Given the dominant role of mesenchymal-osteolineage cells in the regulation of hematopoiesis, we investigated whether menatetrenone alters the hematopoiesis-supportive capability of human bone marrow mesenchymal stromal/stem cells (BM-MSCs). Menatetrenone up-regulated fibronectin protein expression in BM-MSCs without affecting their proliferation and differentiation capabilities. In addition, menatetrenone treatment of BM-MSCs enhanced generation of the CD34+ cell population in co-cultures through acceleration of the cell cycle. This effect was associated with cell-cell interactions mediated by VLA-4 and fibronectin. This proposal was supported by cytokine array and quantitative real-time PCR analyses, in which there were no significant differences between the expression levels of hematopoiesis-associated soluble factors in naïve and menatetrenone-treated BM-MSCs. Profiling of hematopoietic cells in co-cultures with menatetrenone-treated BM-MSCs demonstrated that they included significantly more CD34+CD38+ hematopoietic progenitor cells and cells skewed toward myeloid and megakaryocytic lineages than those in co-cultures with untreated BM-MSCs. Notably, myelodysplastic syndrome-derived cells were induced to undergo apoptosis when co-cultured with BM-MSCs, and this effect was enhanced by menatetrenone. Overall, our findings indicate that pharmacological treatment with menatetrenone bestows a unique hematopoiesis-supportive capability on BM-MSCs, which may contribute to the clinical improvement of cytopenia. ", "subitem_description_type": "Abstract"}]}, "item_4_description_42": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_description": "Journal Article", "subitem_description_type": "Other"}]}, "item_4_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Springer Nature"}]}, "item_4_relation_10": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "32572826", "subitem_relation_type_select": "PMID"}}]}, "item_4_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1007/s12185-020-02916-8"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1007/s12185-020-02916-8", "subitem_relation_type_select": "DOI"}}]}, "item_4_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© Japanese Society of Hematology 2020"}]}, "item_4_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1865-3774", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "FUJISHIRO, Aya"}], "nameIdentifiers": [{"nameIdentifier": "7245", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "IWASA, Masaki"}], "nameIdentifiers": [{"nameIdentifier": "7172", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "0000-0003-4116-7184", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "https://orcid.org/0000-0003-4116-7184"}]}, {"creatorNames": [{"creatorName": "FUJII, Sumie"}], "nameIdentifiers": [{"nameIdentifier": "8008", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MAEKAWA, Taira"}], "nameIdentifiers": [{"nameIdentifier": "8009", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ANDOH, Akira"}], "nameIdentifiers": [{"nameIdentifier": "644", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90252395", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90252395"}, {"nameIdentifier": "0000-0001-8533-2669", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "https://orcid.org/0000-0001-8533-2669"}]}, {"creatorNames": [{"creatorName": "TOHYAMA, Kaoru"}], "nameIdentifiers": [{"nameIdentifier": "8010", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TAKAORI-KONDO, Akifumi"}], "nameIdentifiers": [{"nameIdentifier": "7637", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MIURA, Yasuo"}], "nameIdentifiers": [{"nameIdentifier": "8011", "nameIdentifierScheme": "WEKO"}]}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Hematopoiesis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Menatetrenone", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Mesenchymal stromal/stem cell (MSC)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Microenvironment", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Vitamin K2", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Menatetrenone facilitates hematopoietic cell generation in a manner that is dependent on human bone marrow mesenchymal stromal/stem cells.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Menatetrenone facilitates hematopoietic cell generation in a manner that is dependent on human bone marrow mesenchymal stromal/stem cells."}]}, "item_type_id": "4", "owner": "11", "path": ["228"], "permalink_uri": "http://hdl.handle.net/10422/00012747", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-07-06"}, "publish_date": "2020-07-06", "publish_status": "0", "recid": "3629", "relation": {}, "relation_version_is_last": true, "title": ["Menatetrenone facilitates hematopoietic cell generation in a manner that is dependent on human bone marrow mesenchymal stromal/stem cells."], "weko_shared_id": -1}