{"created":"2023-06-19T10:43:24.256912+00:00","id":3629,"links":{},"metadata":{"_buckets":{"deposit":"5d5e0a65-07c5-4799-ac60-d5bee0f9801e"},"_deposit":{"created_by":11,"id":"3629","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3629"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003629","sets":["30:227:228"]},"author_link":["7172","7245","8011","644","7637","8009","8008","8010"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-06-22","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{},{"bibliographic_title":"International journal of hematology","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤城, 綾"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岩佐, 磨佐紀"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"前川, 平"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安藤, 朗"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Vitamin K2 in the form of menatetrenone has clinical benefits for osteoporosis and cytopenia. Given the dominant role of mesenchymal-osteolineage cells in the regulation of hematopoiesis, we investigated whether menatetrenone alters the hematopoiesis-supportive capability of human bone marrow mesenchymal stromal/stem cells (BM-MSCs). Menatetrenone up-regulated fibronectin protein expression in BM-MSCs without affecting their proliferation and differentiation capabilities. In addition, menatetrenone treatment of BM-MSCs enhanced generation of the CD34+ cell population in co-cultures through acceleration of the cell cycle. This effect was associated with cell-cell interactions mediated by VLA-4 and fibronectin. This proposal was supported by cytokine array and quantitative real-time PCR analyses, in which there were no significant differences between the expression levels of hematopoiesis-associated soluble factors in naïve and menatetrenone-treated BM-MSCs. Profiling of hematopoietic cells in co-cultures with menatetrenone-treated BM-MSCs demonstrated that they included significantly more CD34+CD38+ hematopoietic progenitor cells and cells skewed toward myeloid and megakaryocytic lineages than those in co-cultures with untreated BM-MSCs. Notably, myelodysplastic syndrome-derived cells were induced to undergo apoptosis when co-cultured with BM-MSCs, and this effect was enhanced by menatetrenone. Overall, our findings indicate that pharmacological treatment with menatetrenone bestows a unique hematopoiesis-supportive capability on BM-MSCs, which may contribute to the clinical improvement of cytopenia. ","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"32572826","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1007/s12185-020-02916-8"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s12185-020-02916-8","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Japanese Society of Hematology 2020"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1865-3774","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"FUJISHIRO, Aya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IWASA, Masaki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"FUJII, Sumie"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MAEKAWA, Taira"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ANDOH, Akira"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"TOHYAMA, Kaoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TAKAORI-KONDO, Akifumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MIURA, Yasuo"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hematopoiesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Menatetrenone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mesenchymal stromal/stem cell (MSC)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Microenvironment","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Vitamin K2","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Menatetrenone facilitates hematopoietic cell generation in a manner that is dependent on human bone marrow mesenchymal stromal/stem cells.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Menatetrenone facilitates hematopoietic cell generation in a manner that is dependent on human bone marrow mesenchymal stromal/stem cells."}]},"item_type_id":"4","owner":"11","path":["228"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-07-06"},"publish_date":"2020-07-06","publish_status":"0","recid":"3629","relation_version_is_last":true,"title":["Menatetrenone facilitates hematopoietic cell generation in a manner that is dependent on human bone marrow mesenchymal stromal/stem cells."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T12:16:27.452302+00:00"}