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Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein.
http://hdl.handle.net/10422/00012722
http://hdl.handle.net/10422/00012722844372ae-8e23-44fd-99a6-e77473c6cb72
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JAD-191081 (3.7 MB)
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© 2020 – IOS Press and the authors. All rights reserved
This article is published online with Open Access and distributed under the terms of the Creative Commons Attribution Non-Commercial License (CC BY-NC 4.0). |
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-06-17 | |||||
| タイトル | ||||||
| タイトル | Generation of Transgenic Cynomolgus Monkeys Overexpressing the Gene for Amyloid-β Precursor Protein. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Amyloid-beta protein precursor | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | animal | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | cryopreservation | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | intracytoplasmic sperm injection (ICSI) | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | primates | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | transgenic | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | vitrification | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | zygote | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
SEITA, Yasunari
× SEITA, Yasunari× MORIMURA, Toshifumi× WATANABE, Naoki× IWATANI, Chizuru× TSUCHIYA, Hideaki× NAKAMURA, Shinichiro× SUZUKI, Toshiharu× YANAGISAWA, Daijiro× TSUKIYAMA, Tomoyuki× NAKAYA, Masataka× OKAMURA, Eiichi× MUTO, Masanaga× EMA, Masatsugu× NISHIMURA, Masaki× TOOYAMA, Ikuo |
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| 著者別名 |
清田, 弥寿成
× 清田, 弥寿成× 守村, 敏史× 渡邊, 直希× 岩谷, 千鶴× 土屋, 英明× 中村, 紳一郎× 柳沢, 大治郎× 築山, 智之× 中家, 雅隆× 岡村, 永一× 武藤, 真長× 西村, 正樹× 遠山, 育夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Alzheimer's disease (AD) is the most common cause of dementia and understanding its pathogenesis should lead to improved therapeutic and diagnostic methods. Although several groups have developed transgenic mouse models overexpressing the human amyloid-β precursor protein (APP) gene with AD mutations, with and without presenilin mutations, as well as APP gene knock-in mouse models, these animals display amyloid pathology but do not show neurofibrillary tangles or neuronal loss. This presumably is due to differences between the etiology of the aged-related human disease and the mouse models. Here we report the generation of two transgenic cynomolgus monkeys overexpressing the human gene for APP with Swedish, Artic, and Iberian mutations, and demonstrated expression of gene tagged green fluorescent protein marker in the placenta, amnion, hair follicles, and peripheral blood. We believe that these nonhuman primate models will be very useful to study the pathogenesis of dementia and AD. However, generated Tg monkeys still have some limitations. We employed the CAG promoter, which will promote gene expression in a non-tissue specific manner. Moreover, we used transgenic models but not knock-in models. Thus, the inserted transgene destroys endogenous gene(s) and may affect the phenotype(s). Nevertheless, it will be of great interest to determine whether these Tg monkeys will develop tauopathy and neurodegeneration similar to human AD. | |||||
| 書誌情報 |
en : Journal of Alzheimer's disease : JAD 巻 75, 号 1, p. 45-60, 発行日 2020-06 |
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| 出版者 | ||||||
| 出版者 | IOS Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1875-8908 | |||||
| PMID | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 32250299 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.3233/jad-191081 | |||||
| 関連名称 | 10.3233/JAD-191081 | |||||
| 権利 | ||||||
| 権利情報 | 2020 – IOS Press and the authors. | |||||
| 権利 | ||||||
| 権利情報 | This article is published online with Open Access and distributed under the terms of the Creative Commons Attribution Non-Commercial License (CC BY-NC 4.0). | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal Article | |||||
