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Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body.
http://hdl.handle.net/10422/11674
http://hdl.handle.net/10422/11674becca200-da7b-4079-8072-bfe361b2c8d4
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
srep24868.pdf (2.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-05-13 | |||||
| タイトル | ||||||
| タイトル | Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Animal disease model | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Stem-cell research | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
SEITA, Yasunari
× SEITA, Yasunari× TSUKIYAMA, Tomoyuki× IWATANI, Chizuru× TSUCHIYA, Hideaki× MATSUSHITA, Jun× AZAMI, Takuya× OKAHARA, Junko× NAKAMURA, Shinichiro× HAYASHI, Yoshitaka× HITOSHI, Seiji× ITOH, Yasushi× IMAMURA, Takeshi× NISHIMURA, Masaki× TOOYAMA, Ikuo× MIYOSHI, Hiroyuki× SAITOU, Mitinori× OGASAWARA, Kazumasa× SASAKI, Erika× EMA, Masatsugu |
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| 著者別名 |
清田, 弥寿成
× 清田, 弥寿成× 築山, 智之× 岩谷, 千鶴× 土屋, 英明× 岡原, 純子× 中村, 紳一郎× 林, 義剛× 等, 誠司× 伊藤, 靖× 西村, 正樹× 遠山, 育夫× 小笠原, 一誠× 依馬, 正次 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Nonhuman primates are valuable for human disease modelling, because rodents poorly recapitulate some human diseases such as Parkinson's disease and Alzheimer's disease amongst others. Here, we report for the first time, the generation of green fluorescent protein (GFP) transgenic cynomolgus monkeys by lentivirus infection. Our data show that the use of a human cytomegalovirus immediate-early enhancer and chicken beta actin promoter (CAG) directed the ubiquitous expression of the transgene in cynomolgus monkeys. We also found that injection into mature oocytes before fertilization achieved homogenous expression of GFP in each tissue, including the amnion, and fibroblasts, whereas injection into fertilized oocytes generated a transgenic cynomolgus monkey with mosaic GFP expression. Thus, the injection timing was important to create transgenic cynomolgus monkeys that expressed GFP homogenously in each of the various tissues. The strategy established in this work will be useful for the generation of transgenic cynomolgus monkeys for transplantation studies as well as biomedical research. | |||||
| 書誌情報 |
Scientific Reports 巻 6, 発行日 2016-04-25 |
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| 出版者 | ||||||
| 出版者 | Nature Publishing Group | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2045-2322 | |||||
| PMID | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 27109065 | |||||
| PMCID | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/pmc/articles/pmc4843004/ | |||||
| 関連名称 | PMC4843004 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1038/srep24868 | |||||
| 関連名称 | 10.1038/srep24868 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ||||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal Article | |||||
