@article{oai:shiga-med.repo.nii.ac.jp:00002915,
 author = {清田, 弥寿成 and 築山, 智之 and 岩谷, 千鶴 and 土屋, 英明 and 岡原, 純子 and 中村, 紳一郎 and 林, 義剛 and 等, 誠司 and 伊藤, 靖 and 西村, 正樹 and 遠山, 育夫 and 小笠原, 一誠 and 依馬, 正次 and SEITA, Yasunari and TSUKIYAMA, Tomoyuki and IWATANI, Chizuru and TSUCHIYA, Hideaki and MATSUSHITA, Jun and AZAMI, Takuya and OKAHARA, Junko and NAKAMURA, Shinichiro and HAYASHI, Yoshitaka and HITOSHI, Seiji and ITOH, Yasushi and IMAMURA, Takeshi and NISHIMURA, Masaki and TOOYAMA, Ikuo and MIYOSHI, Hiroyuki and SAITOU, Mitinori and OGASAWARA, Kazumasa and SASAKI, Erika and EMA, Masatsugu},
 journal = {Scientific Reports},
 month = {Apr},
 note = {pdf, Nonhuman primates are valuable for human disease modelling, because rodents poorly recapitulate some human diseases such as Parkinson's disease and Alzheimer's disease amongst others. Here, we report for the first time, the generation of green fluorescent protein (GFP) transgenic cynomolgus monkeys by lentivirus infection. Our data show that the use of a human cytomegalovirus immediate-early enhancer and chicken beta actin promoter (CAG) directed the ubiquitous expression of the transgene in cynomolgus monkeys. We also found that injection into mature oocytes before fertilization achieved homogenous expression of GFP in each tissue, including the amnion, and fibroblasts, whereas injection into fertilized oocytes generated a transgenic cynomolgus monkey with mosaic GFP expression. Thus, the injection timing was important to create transgenic cynomolgus monkeys that expressed GFP homogenously in each of the various tissues. The strategy established in this work will be useful for the generation of transgenic cynomolgus monkeys for transplantation studies as well as biomedical research., Journal Article},
 title = {Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body.},
 volume = {6},
 year = {2016}
}