{"created":"2023-06-19T10:42:52.459506+00:00","id":2915,"links":{},"metadata":{"_buckets":{"deposit":"974ae344-9c85-4cd1-8d9c-31b1226c1d5d"},"_deposit":{"created_by":3,"id":"2915","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2915"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00002915","sets":["19:82:83"]},"author_link":["6523","6393","7664","1844","3112","4059","6403","6400","5888","1961","2545","6410","6409","1957","6506","1971","4000","6412","6470"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04-25","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"清田, 弥寿成"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"築山, 智之"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"岩谷, 千鶴"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"土屋, 英明"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"岡原, 純子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"中村, 紳一郎"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"林, 義剛"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"等, 誠司"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"伊藤, 靖"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"西村, 正樹"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"遠山, 育夫"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"小笠原, 一誠"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"依馬, 正次"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nonhuman primates are valuable for human disease modelling, because rodents poorly recapitulate some human diseases such as Parkinson's disease and Alzheimer's disease amongst others. Here, we report for the first time, the generation of green fluorescent protein (GFP) transgenic cynomolgus monkeys by lentivirus infection. Our data show that the use of a human cytomegalovirus immediate-early enhancer and chicken beta actin promoter (CAG) directed the ubiquitous expression of the transgene in cynomolgus monkeys. We also found that injection into mature oocytes before fertilization achieved homogenous expression of GFP in each tissue, including the amnion, and fibroblasts, whereas injection into fertilized oocytes generated a transgenic cynomolgus monkey with mosaic GFP expression. Thus, the injection timing was important to create transgenic cynomolgus monkeys that expressed GFP homogenously in each of the various tissues. The strategy established in this work will be useful for the generation of transgenic cynomolgus monkeys for transplantation studies as well as biomedical research.","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Publishing Group"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"27109065","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1038/srep24868"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/srep24868","subitem_relation_type_select":"DOI"}}]},"item_4_relation_13":{"attribute_name":"PMCID","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"PMC4843004"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc4843004/","subitem_relation_type_select":"URI"}}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SEITA, Yasunari"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"TSUKIYAMA, Tomoyuki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"IWATANI, Chizuru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUCHIYA, Hideaki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"MATSUSHITA, Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"AZAMI, Takuya"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"OKAHARA, Junko"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"NAKAMURA, Shinichiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"HAYASHI, Yoshitaka"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"HITOSHI, Seiji"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"ITOH, Yasushi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"IMAMURA, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NISHIMURA, Masaki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"TOOYAMA, Ikuo"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"MIYOSHI, Hiroyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAITOU, Mitinori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OGASAWARA, Kazumasa"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"SASAKI, Erika"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"EMA, Masatsugu"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-17"}],"displaytype":"detail","filename":"srep24868.pdf","filesize":[{"value":"2.6 MB"}],"format":"application/pdf","license_note":"This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"srep24868.pdf","url":"https://shiga-med.repo.nii.ac.jp/record/2915/files/srep24868.pdf"},"version_id":"53ad923d-108e-4b16-9393-d7e31551c906"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Animal disease model","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Stem-cell research","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body."}]},"item_type_id":"4","owner":"3","path":["83"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-05-13"},"publish_date":"2016-05-13","publish_status":"0","recid":"2915","relation_version_is_last":true,"title":["Generation of transgenic cynomolgus monkeys that express green fluorescent protein throughout the whole body."],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T11:16:34.725207+00:00"}