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Characterization and functional role of rapid- and slow-activating delayed rectifier K + currents in atrioventricular node cells of guinea pigs.
http://hdl.handle.net/10422/00013121
http://hdl.handle.net/10422/00013121cf4c3619-9e65-4ff2-9286-279ee021b6e5
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-11-09 | |||||
| タイトル | ||||||
| タイトル | Characterization and functional role of rapid- and slow-activating delayed rectifier K + currents in atrioventricular node cells of guinea pigs. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Atrioventricular node | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | I Kr | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | I Ks | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Spontaneous activity | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Sympathetic stimulation | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
YUASA, Mayumi
× YUASA, Mayumi× KOJIMA, Akiko× Mi, Xinya× DING, Wei-guang× OMATSU-KANBE, Mariko× KITAGAWA, Hirotoshi× MATSUURA, Hiroshi |
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| 著者別名 |
湯浅, 真由美
× 湯浅, 真由美× 小嶋, 亜希子× 林, 維光× 尾松, 万里子× 北川, 裕利× 松浦, 博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The atrioventricular (AV) node is the only conduction pathway where electrical impulse can pass from atria to ventricles and exhibits spontaneous automaticity. This study examined the function of the rapid- and slow-activating delayed rectifier K+ currents (IKr and IKs) in the regulation of AV node automaticity. Isolated AV node cells from guinea pigs were current- and voltage-clamped to record the action potentials and the IKr and IKs current. The expression of IKr or IKs was confirmed in the AV node cells by immunocytochemistry, and the positive signals of both channels were localized mainly on the cell membrane. The basal spontaneous automaticity was equally reduced by E4031 and HMR-1556, selective blockers of IKr and IKs, respectively. The nonselective β-adrenoceptor agonist isoproterenol markedly increased the firing rate of action potentials. In the presence of isoproterenol, the firing rate of action potentials was more effectively reduced by the IKs inhibitor HMR-1556 than by the IKr inhibitor E4031. Both E4031 and HMR-1556 prolonged the action potential duration and depolarized the maximum diastolic potential under basal and β-adrenoceptor–stimulated conditions. IKr was not significantly influenced by β-adrenoceptor stimulation, but IKs was concentration-dependently enhanced by isoproterenol (EC50: 15 nM), with a significant negative voltage shift in the channel activation. These findings suggest that both the IKr and IKs channels might exert similar effects on regulating the repolarization process of AV node action potentials under basal conditions; however, when the β-adrenoceptor is activated, IKs modulation may become more important. | |||||
| 書誌情報 |
en : Pflugers Archiv European Journal of Physiology 発行日 2021-10-26 |
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| 出版者 | ||||||
| 出版者 | Springer Science and Business Media Deutschland GmbH | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0031-6768 | |||||
| PMID | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 34704178 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s00424-021-02617-z | |||||
| 関連名称 | 10.1007/s00424-021-02617-z | |||||
| 権利 | ||||||
| 権利情報 | © 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal Article | |||||
