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Host factors influence Barrett's carcinogenesis: findings from a mouse gastroduodenal reflux model.
http://hdl.handle.net/10422/00012796
http://hdl.handle.net/10422/00012796a4f1f827-ac1e-452a-94eb-e9ef5c3c51d4
名前 / ファイル | ライセンス | アクション |
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s10388-019-00660-5 (1.3 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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公開日 | 2020-09-17 | |||||||
タイトル | ||||||||
タイトル | Host factors influence Barrett's carcinogenesis: findings from a mouse gastroduodenal reflux model. | |||||||
言語 | en | |||||||
言語 | ||||||||
言語 | eng | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Barrett’s esophagus | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Bile acids | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Carcinogenesis | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Mouse | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Taurine conjugate | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
資源タイプ | doctoral thesis | |||||||
アクセス権 | ||||||||
アクセス権 | open access | |||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
著者 |
KANAI, Shunpei
× KANAI, Shunpei
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著者別名 |
金井, 俊平
× 金井, 俊平
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抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | Background: Rat gastroduodenal reflux models have been used for analyzing Barrett's carcinogenesis. Mice seem to be more useful than rats for studies targeting genes. |
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言語 | en | |||||||
抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | Methods: We induced gastroduodenal contents reflux by esophagojejunostomy using C57BL/6J mice. Mice were divided into a standard diet and high-fat diet groups and kept for 60 weeks. Bile was sampled from the gallbladder to analyze bile acid fractions, and the esophagus was removed for a histological investigation. Human esophagogastric junction adenocarcinoma cells (OE19) were exposed to taurocholic acid (TCA), after which cell proliferative activity was measured. Rat esophageal cancer cell lines, ESCC-DR and ESCC-DRtca with higher malignant potential induced by continuous TCA exposure, were used to perform comprehensive genetic analysis (CGH). |
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言語 | en | |||||||
抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | Results: Barrett's epithelium onset occurred in all mice, and no differences in histological changes were noted between the standard diet and high-fat diet groups. However, no development of adenocarcinoma was noted. Most of the mouse bile acid was taurine conjugates. In the experiment using OE-19 cells, TCA promotes cell proliferation in a dose-dependent manner. Array CGH analysis revealed a large number of chromosomal abnormalities in the ESCC-DR, in addition to genetic abnormalities such as in the UGT2B gene, the substrate of which is bile acid. TCA administration resulted in more chromosomal abnormalities being detected. |
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言語 | en | |||||||
抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | Conclusions: We showed the effects of TCA in cancer progression in vitro. However, Barrett's adenocarcinoma onset rates differ between mice and rats despite undergoing similar reflux stimulation including taurine-conjugated bile acids being detected in mouse bile juice. These results suggest that host factors seem to influence Barrett's carcinogenesis. |
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言語 | en | |||||||
学位名 | ||||||||
言語 | ja | |||||||
学位名 | 博士(医学) | |||||||
学位授与機関 | ||||||||
学位授与機関識別子Scheme | kakenhi | |||||||
学位授与機関識別子 | 14202 | |||||||
言語 | ja | |||||||
学位授与機関名 | 滋賀医科大学 | |||||||
学位授与年度 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | 令和元年度 | |||||||
言語 | ja | |||||||
学位授与年月日 | ||||||||
学位授与年月日 | 2019-09-11 | |||||||
学位授与番号 | ||||||||
学位授与番号 | 甲第849号 | |||||||
書誌情報 |
en : Esophagus 巻 16, 号 3, p. 264--271, 発行日 2019-02-21 |
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出版者 | ||||||||
出版者 | Springer Tokyo | |||||||
言語 | en | |||||||
権利 | ||||||||
権利情報 | © The Author(s) 2019 | |||||||
著者版フラグ | ||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
ISSN | ||||||||
収録物識別子タイプ | PISSN | |||||||
収録物識別子 | 1612-9059 | |||||||
PMID | ||||||||
識別子タイプ | PMID | |||||||
関連識別子 | 30790117 | |||||||
DOI | ||||||||
識別子タイプ | DOI | |||||||
関連識別子 | https://doi.org/10.1007/s10388-019-00660-5 | |||||||
関連名称 | 10.1007/s10388-019-00660-5 | |||||||
資源タイプ | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | Thesis or Dissertation | |||||||
関係URI | ||||||||
識別子タイプ | HDL | |||||||
関連識別子 | http://hdl.handle.net/10422/00012664 | |||||||
関連名称 | 博士論文要旨 |