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A simple antegrade perfusion method for isolating viable single cardiomyocytes from neonatal to aged mice.
http://hdl.handle.net/10422/00012460
http://hdl.handle.net/10422/0001246083e45a28-c0d1-4ad7-80ce-8eb246fc0528
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
phy2.13688 (2.8 MB)
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©2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-11-06 | |||||
| タイトル | ||||||
| タイトル | A simple antegrade perfusion method for isolating viable single cardiomyocytes from neonatal to aged mice. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Antegrade perfusion | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | isolation of cardiomyocytes | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | mouse heart | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
尾松, 万里子
× 尾松, 万里子× Yoshioka, Kengo× Fukunaga, Ryo× 狭川, 浩規× 松浦, 博 |
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| 著者別名 |
尾松, 万里子
× 尾松, 万里子× 吉岡, 賢吾× 福永, 諒× 狭川, 浩規× 松浦, 博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of this study was to establish a simple and reproducible antegrade perfusion method for isolating single viable mouse heart cells and to determine the standard practical protocols that are appropriate for mice of various ages. Antegrade perfusion was performed by injecting perfusate from near the apex of the left ventricle of the excised heart, the aorta of which was clamped, using an infusion pump. This could thoroughly perfuse the myocardium through the coronary circulation. All procedures were carried out on a prewarmed heater mat under a microscope, which allows for the processes of injection and perfusion to be monitored. With appropriate adjustment of the size of the injection needle, the composition and amount of enzyme solution and the perfusion flow rate, this antegrade perfusion method could be applied to the hearts of neonatal to aged mice. We examined the morphological characteristics and electrophysiological properties of the isolated ventricular and atrial myocytes and found that these cells were mostly identical to those obtained with the traditional Langendorff-based retrograde perfusion method. Interstitial nonmyocytes, such as cardiac progenitor cells, were also isolated simultaneously from the supernatant fraction of the centrifugation, similar to the retrograde perfusion method. The results suggest that single heart cells can be well isolated with high degree of quality by the present antegrade perfusion method, regardless of the age of the mouse. | |||||
| 書誌情報 |
Physiological Reports 巻 6, 号 9, p. e13688, 発行日 2018-05 |
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| 出版者 | ||||||
| 出版者 | American Physiological Society | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2051-817X | |||||
| PMID | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 29696821 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.14814/phy2.13688 | |||||
| 権利 | ||||||
| 権利情報 | ©2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal Article | |||||
