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β-Adrenergic stimulation of short-circuit currents in human intestinal epithelial caco-2 cells
http://hdl.handle.net/10422/116
http://hdl.handle.net/10422/11610747f81-903e-4f99-9930-b535842805a8
名前 / ファイル | ライセンス | アクション |
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jsums17p005.pdf (553.3 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-04-12 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | β-Adrenergic stimulation of short-circuit currents in human intestinal epithelial caco-2 cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
その他のタイトル | ||||||
その他のタイトル | ヒト腸管上皮細胞Caco-2における短絡電流に対するβアゴニストの作用 | |||||
言語 | ja | |||||
その他のタイトル | ||||||
その他のタイトル | ヒト チョウカン ジョウヒ サイボウ Caco-2 ニ オケル タンラク デンリュウ ニ タイスル β アゴニスト ノ サヨウ | |||||
言語 | ja-Kana | |||||
著者 |
HIRANO, Takashi
× HIRANO, Takashi× OHARA, Akito× SAEKI, Yukikazu× FUJIMURA, Masaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To investigate the effects of epinephrine on ion transport of intestinal epithelia, short-circuit currents(Isc) in monolayers of Caco-2 cells grown on permeable membrane supports were measured in theUssing chamber. The application of epinephrine to the basolateral solution produced a transient increase ofIsc (maximal currents induced by 5 μM and more of epinephrine). The value of the peak current was 3.4 μA/cm2. Isoproterenol (a β-agonist) increased Isc in a manner analogous to epinephrine application, but phenylephrine(an α1-agonist) induced no Isc change. Clonidine (an α2-agonist) transiently decreased Isc by about0.8 μA/cm2 at a high dose of 100 μM. The Isc increase induced by epinephrine or isoproterenol was mimickedby a membrane-permeable cyclic AMP analogue, dibutyryl cyclic AMP, or a calcium ionophore, A23187. Verapamil, a calcium channel blocker, eliminated the epinephrine-induced Isc increase, and 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), a chloride channel blocker, reduced the Isc increase. These observationssuggest that in Caco-2 epithelia (i) epinephrine induces Isc increase via β-adrenergic receptors,and (ii) the epinephrine-induced Isc responses have at least two components, namely, verapamil-sensitiveCa2+ and NPPB-sensitive Cl- transports. | |||||
言語 | en | |||||
書誌情報 |
ja : 滋賀医科大学雑誌 巻 17, p. 5-13, 発行日 2002-02 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 滋賀医科大学雑誌刊行会 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10044327 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |