{"created":"2023-06-19T10:40:35.101441+00:00","id":89,"links":{},"metadata":{"_buckets":{"deposit":"8ab300b8-f468-4149-9ae9-d0275f3ca6ca"},"_deposit":{"created_by":3,"id":"89","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"89"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00000089","sets":["6:7:158"]},"author_link":["277","278","1013","176"],"control_number":"89","item_3_alternative_title_18":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ヒト腸管上皮細胞Caco-2における短絡電流に対するβアゴニストの作用","subitem_alternative_title_language":"ja"},{"subitem_alternative_title":"ヒト チョウカン ジョウヒ サイボウ Caco-2 ニ オケル タンラク デンリュウ ニ タイスル β アゴニスト ノ サヨウ","subitem_alternative_title_language":"ja-Kana"}]},"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-02","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"13","bibliographicPageStart":"5","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"滋賀医科大学雑誌","bibliographic_titleLang":"ja"}]}]},"item_3_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To investigate the effects of epinephrine on ion transport of intestinal epithelia, short-circuit currents(Isc) in monolayers of Caco-2 cells grown on permeable membrane supports were measured in theUssing chamber. The application of epinephrine to the basolateral solution produced a transient increase ofIsc (maximal currents induced by 5 μM and more of epinephrine). The value of the peak current was 3.4 μA/cm2. Isoproterenol (a β-agonist) increased Isc in a manner analogous to epinephrine application, but phenylephrine(an α1-agonist) induced no Isc change. Clonidine (an α2-agonist) transiently decreased Isc by about0.8 μA/cm2 at a high dose of 100 μM. The Isc increase induced by epinephrine or isoproterenol was mimickedby a membrane-permeable cyclic AMP analogue, dibutyryl cyclic AMP, or a calcium ionophore, A23187. Verapamil, a calcium channel blocker, eliminated the epinephrine-induced Isc increase, and 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB), a chloride channel blocker, reduced the Isc increase. These observationssuggest that in Caco-2 epithelia (i) epinephrine induces Isc increase via β-adrenergic receptors,and (ii) the epinephrine-induced Isc responses have at least two components, namely, verapamil-sensitiveCa2+ and NPPB-sensitive Cl- transports.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"滋賀医科大学雑誌刊行会","subitem_publisher_language":"ja"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10044327","subitem_source_identifier_type":"NCID"}]},"item_3_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"HIRANO, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHARA, Akito","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SAEKI, Yukikazu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"FUJIMURA, Masaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"jsums17p005.pdf","filesize":[{"value":"553.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jsums17p005.pdf","objectType":"fulltext","url":"https://shiga-med.repo.nii.ac.jp/record/89/files/jsums17p005.pdf"},"version_id":"e643b606-3334-422a-b56f-baec177f63aa"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"β-Adrenergic stimulation of short-circuit currents in human intestinal epithelial caco-2 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"β-Adrenergic stimulation of short-circuit currents in human intestinal epithelial caco-2 cells","subitem_title_language":"en"}]},"item_type_id":"3","owner":"3","path":["158"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-04-12"},"publish_date":"2016-04-12","publish_status":"0","recid":"89","relation_version_is_last":true,"title":["β-Adrenergic stimulation of short-circuit currents in human intestinal epithelial caco-2 cells"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-02-15T01:35:16.297071+00:00"}