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Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice.
http://hdl.handle.net/10422/00013326
http://hdl.handle.net/10422/00013326240955b1-6675-4868-8fbd-5222d1c5c634
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
expanim.21-0111 (2.2 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2022-06-14 | |||||||
| タイトル | ||||||||
| タイトル | Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice. | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | leucine-rich repeat containing 8A | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | myometrium | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | pregnancy | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | swelling-induced Cl–current | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | volume-regulated anion channel | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
YAMADA, Kazutaka
× YAMADA, Kazutaka
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| 著者別名 |
山田, 一貴
× 山田, 一貴
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Pregnancy causes changes in the uterus, such as increased cell volume and altered water content. However, the mechanisms that protect the structure and maintain the function of uterine smooth muscle cells against these changes during pregnancy have not been clarified. This study focused on the volume-regulated anion channel (VRAC), which opens with cell swelling under low osmotic pressure and releases Cl– ions and various organic osmolytes to resist cell swelling and regulates a wide range of biological processes such as cell death. In this study, myometrial smooth muscle (MSM) tissues and cells (MSMCs) were collected from non-pregnant and pregnant mice. Using western blotting and immunocytochemistry, leucine-rich repeat containing protein 8A (LRRC8A), an essential membrane protein that constitutes part of the VRAC, was determined to be diffused throughout MSMCs including in the cell membrane. Patch-clamp experiments were performed to investigate the electrophysiology of swelling-induced Cl– currents (ICl, swell) mediated by the VRAC. No significant changes between non-pregnancy and pregnancy groups were observed in either the expression density of LRRC8A or the current density of ICl, swell, however the presence of LRRC8A on the cell membrane was significantly increased in the third trimester of pregnancy compared to the non-pregnancy. This study suggests that the VRAC may play a role, such as maintaining cellular homeostasis in the pregnant MSM. | |||||||
| 言語 | en | |||||||
| 学位名 | ||||||||
| 言語 | ja | |||||||
| 学位名 | 博士（医学） | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 14202 | |||||||
| 言語 | ja | |||||||
| 学位授与機関名 | 滋賀医科大学 | |||||||
| 学位授与年度 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 令和3年度 | |||||||
| 言語 | ja | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2022-03-10 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲第925号 | |||||||
| 書誌情報 |
en : Experimental animals 巻 71, 号 2, p. 123-130, 発行日 2022-05-20 |
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| 出版者 | ||||||||
| 出版者 | Japanese Association for Laboratory Animal Science | |||||||
| 言語 | en | |||||||
| 権利 | ||||||||
| 権利情報 | ©2022 Japanese Association for Laboratory Animal Science | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | ||||||||
| 著者版フラグ | ||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | EISSN | |||||||
| 収録物識別子 | 1881-7122 | |||||||
| PMID | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | 34789619 | |||||||
| DOI | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | https://doi.org/10.1538/expanim.21-0111 | |||||||
| 関連名称 | 10.1538/expanim.21-0111 | |||||||
| 資源タイプ | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Thesis or Dissertation | |||||||
| 関係URI | ||||||||
| 識別子タイプ | HDL | |||||||
| 関連識別子 | http://hdl.handle.net/10422/00013327 | |||||||
| 関連名称 | 博士論文要旨 | |||||||
