{"created":"2023-06-19T10:43:53.545781+00:00","id":4240,"links":{},"metadata":{"_buckets":{"deposit":"f384a0f2-856c-430c-9fc3-f83756854c9c"},"_deposit":{"created_by":11,"id":"4240","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"4240"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00004240","sets":["10:37"]},"author_link":[],"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-05-20","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"130","bibliographicPageStart":"123","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"Experimental animals","bibliographic_titleLang":"en"}]}]},"item_6_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山田, 一貴","creatorNameLang":"ja"}]}]},"item_6_date_granted_70":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2022-03-10"}]},"item_6_degree_grantor_68":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"滋賀医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14202","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_67":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_6_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"pdf","subitem_description_type":"Other"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Pregnancy causes changes in the uterus, such as increased cell volume and altered water content. However, the mechanisms that protect the structure and maintain the function of uterine smooth muscle cells against these changes during pregnancy have not been clarified. This study focused on the volume-regulated anion channel (VRAC), which opens with cell swelling under low osmotic pressure and releases Cl– ions and various organic osmolytes to resist cell swelling and regulates a wide range of biological processes such as cell death. In this study, myometrial smooth muscle (MSM) tissues and cells (MSMCs) were collected from non-pregnant and pregnant mice. Using western blotting and immunocytochemistry, leucine-rich repeat containing protein 8A (LRRC8A), an essential membrane protein that constitutes part of the VRAC, was determined to be diffused throughout MSMCs including in the cell membrane. Patch-clamp experiments were performed to investigate the electrophysiology of swelling-induced Cl– currents (ICl, swell) mediated by the VRAC. No significant changes between non-pregnancy and pregnancy groups were observed in either the expression density of LRRC8A or the current density of ICl, swell, however the presence of LRRC8A on the cell membrane was significantly increased in the third trimester of pregnancy compared to the non-pregnancy. This study suggests that the VRAC may play a role, such as maintaining cellular homeostasis in the pregnant MSM.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Thesis or Dissertation","subitem_description_type":"Other"}]},"item_6_description_69":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"令和3年度","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_71":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第925号"}]},"item_6_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Association for Laboratory Animal Science","subitem_publisher_language":"en"}]},"item_6_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"34789619","subitem_relation_type_select":"PMID"}}]},"item_6_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1538/expanim.21-0111"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1538/expanim.21-0111","subitem_relation_type_select":"DOI"}}]},"item_6_relation_48":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"博士論文要旨"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10422/00013327","subitem_relation_type_select":"HDL"}}]},"item_6_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©2022 Japanese Association for Laboratory Animal Science"}]},"item_6_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1881-7122","subitem_source_identifier_type":"EISSN"}]},"item_6_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"YAMADA, Kazutaka","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-06-14"}],"displaytype":"detail","filename":"expanim.21-0111.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensefree":"This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives\n(by-nc-nd) License \n<http://creativecommons.org/licenses/by-nc-nd/4.0/>.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"expanim.21-0111","objectType":"fulltext","url":"https://shiga-med.repo.nii.ac.jp/record/4240/files/expanim.21-0111.pdf"},"version_id":"dc16d957-d5e9-4111-96e7-7bb0cf96e26f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"leucine-rich repeat containing 8A","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"myometrium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pregnancy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"swelling-induced Cl–current","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"volume-regulated anion channel","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice.","subitem_title_language":"en"}]},"item_type_id":"6","owner":"11","path":["37"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-06-14"},"publish_date":"2022-06-14","publish_status":"0","recid":"4240","relation_version_is_last":true,"title":["Expression and functional maintenance of volume-regulated anion channels in myometrial smooth muscles of pregnant mice."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-09-15T05:18:51.143457+00:00"}