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The Effect of Ethanol on Disassembly of Amyloid-β1-42 Pentamer Revealed by Atomic Force Microscopy and Gel Electrophoresis.
http://hdl.handle.net/10422/00013190
http://hdl.handle.net/10422/00013190d7348d47-f756-4458-8147-fbb490214872
名前 / ファイル | ライセンス | アクション |
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ijms23020889 (6.6 MB)
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This article is an open access article distributed under the terms and
conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-01-24 | |||||
タイトル | ||||||
タイトル | The Effect of Ethanol on Disassembly of Amyloid-β1-42 Pentamer Revealed by Atomic Force Microscopy and Gel Electrophoresis. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | amyloid-β | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | oligomer | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | atomic force microscopy | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | gel electrophoresis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Gaussian fitting | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
MATSUI, Atsuya
× MATSUI, Atsuya× BELLIER, Jean-Pierre× KANAI, Takeshi× SATOOKA, Hiroki× NAKANISHI, Akio× TERADA, Tsukasa× ISHIBE, Takafumi× NAKAMURA, Yoshiaki× TAGUCHI, Hiroyasu× NARUSE, Nobuyasu× MERA, Yutaka |
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著者別名 |
松井, 温哉
× 松井, 温哉× 金井, 岳志× 里岡, 大樹× 中西, 章夫× 田口, 弘康× 成瀬, 延康× 目良, 裕 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The most common type of dementia, Alzheimer's disease, is associated with senile plaques formed by the filamentous aggregation of hydrophobic amyloid-β (Aβ) in the brains of patients. Small oligomeric assemblies also occur and drugs and chemical compounds that can interact with such assemblies have attracted much attention. However, these compounds need to be solubilized in appropriate solvents, such as ethanol, which may also destabilize their protein structures. As the impact of ethanol on oligomeric Aβ assembly is unknown, we investigated the effect of various concentrations of ethanol (0 to 7.2 M) on Aβ pentameric assemblies (Aβp) by combining blue native-PAGE (BN-PAGE) and ambient air atomic force microscopy (AFM). This approach was proven to be very convenient and reliable for the quantitative analysis of Aβ assembly. The Gaussian analysis of the height histogram obtained from the AFM images was correlated with band intensity on BN-PAGE for the quantitative estimation of Aβp. Our observations indicated up to 1.4 M (8.3%) of added ethanol can be used as a solvent/vehicle without quantitatively affecting Aβ pentamer stability. Higher concentration induced significant destabilization of Aβp and eventually resulted in the complete disassembly of Aβp. | |||||
書誌情報 |
en : International journal of molecular sciences 巻 23, 号 2, 発行日 2022-01-14 |
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出版者 | ||||||
出版者 | MDPI | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1422-0067 | |||||
PMID | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 35055076 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3390/ijms23020889 | |||||
関連名称 | 10.3390/ijms23020889 | |||||
権利 | ||||||
権利情報 | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | ||||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal Article |