{"created":"2023-06-19T10:43:46.899132+00:00","id":4094,"links":{},"metadata":{"_buckets":{"deposit":"a5046f4a-c7b4-4b05-896d-95025937b33b"},"_deposit":{"created_by":11,"id":"4094","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"4094"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00004094","sets":["19:34:35","30:389:390"]},"author_link":["4013","8816","6573","6484","1137","7872","8318","8815","8814","8813","8817"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-01-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicVolumeNumber":"23","bibliographic_titles":[{},{"bibliographic_title":"International journal of molecular sciences","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松井, 温哉"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"金井, 岳志"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"里岡, 大樹"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"中西, 章夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田口, 弘康"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"成瀬, 延康"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"目良, 裕"}],"nameIdentifiers":[{},{}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The most common type of dementia, Alzheimer's disease, is associated with senile plaques formed by the filamentous aggregation of hydrophobic amyloid-β (Aβ) in the brains of patients. Small oligomeric assemblies also occur and drugs and chemical compounds that can interact with such assemblies have attracted much attention. However, these compounds need to be solubilized in appropriate solvents, such as ethanol, which may also destabilize their protein structures. As the impact of ethanol on oligomeric Aβ assembly is unknown, we investigated the effect of various concentrations of ethanol (0 to 7.2 M) on Aβ pentameric assemblies (Aβp) by combining blue native-PAGE (BN-PAGE) and ambient air atomic force microscopy (AFM). This approach was proven to be very convenient and reliable for the quantitative analysis of Aβ assembly. The Gaussian analysis of the height histogram obtained from the AFM images was correlated with band intensity on BN-PAGE for the quantitative estimation of Aβp. Our observations indicated up to 1.4 M (8.3%) of added ethanol can be used as a solvent/vehicle without quantitatively affecting Aβ pentamer stability. Higher concentration induced significant destabilization of Aβp and eventually resulted in the complete disassembly of Aβp. ","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"35055076","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.3390/ijms23020889"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/ijms23020889","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 by the authors. Licensee MDPI, Basel, Switzerland."}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MATSUI, Atsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"BELLIER, Jean-Pierre"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"KANAI, Takeshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SATOOKA, Hiroki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"NAKANISHI, Akio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TERADA, Tsukasa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ISHIBE, Takafumi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"NAKAMURA, Yoshiaki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"TAGUCHI, Hiroyasu"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"NARUSE, Nobuyasu"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"MERA, Yutaka"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-24"}],"displaytype":"detail","filename":"ijms23020889.pdf","filesize":[{"value":"6.6 MB"}],"format":"application/pdf","license_note":"This article is an open access article distributed under the terms and\nconditions of the Creative Commons Attribution (CC BY) license \n(https://creativecommons.org/licenses/by/4.0/).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ijms23020889","url":"https://shiga-med.repo.nii.ac.jp/record/4094/files/ijms23020889.pdf"},"version_id":"3da28623-d4b0-4942-ba4a-4c31af53de62"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"amyloid-β","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"oligomer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"atomic force microscopy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gel electrophoresis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gaussian fitting","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The Effect of Ethanol on Disassembly of Amyloid-β1-42 Pentamer Revealed by Atomic Force Microscopy and Gel Electrophoresis.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The Effect of Ethanol on Disassembly of Amyloid-β1-42 Pentamer Revealed by Atomic Force Microscopy and Gel Electrophoresis."}]},"item_type_id":"4","owner":"11","path":["35","390"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-24"},"publish_date":"2022-01-24","publish_status":"0","recid":"4094","relation_version_is_last":true,"title":["The Effect of Ethanol on Disassembly of Amyloid-β1-42 Pentamer Revealed by Atomic Force Microscopy and Gel Electrophoresis."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T11:19:12.606247+00:00"}