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An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice.
http://hdl.handle.net/10422/00013028
http://hdl.handle.net/10422/000130284c29106e-8480-47d4-99d2-3f0a700e82c4
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-06-22 | |||||
| タイトル | ||||||
| タイトル | An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
OMATSU-KANBE, Mariko
× OMATSU-KANBE, Mariko× FUKUNAGA, Ryo× MI, Xinya× MATSUURA, Hiroshi |
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| 著者別名 |
尾松, 万里子
× 尾松, 万里子× 福永, 諒× 松浦, 博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In basic research using mouse heart, isolating viable individual cardiomyocytes is a crucial technical step to overcome. Traditionally, isolating cardiomyocytes from rabbits, guinea pigs or rats has been performed via retrograde perfusion of the heart with enzymes using a Langendorff apparatus. However, a high degree of skill is required when this method is used with a small mouse heart. An antegrade perfusion method that does not use a Langendorff apparatus was recently reported for the isolation of mouse cardiomyocytes. We herein report a complete protocol for the improved antegrade perfusion of the excised heart to isolate individual heart cells from adult mice (8 - 108 weeks old). Antegrade perfusion is performed by injecting perfusate near the apex of the left ventricle of the excised heart, the aorta of which was clamped, using an infusion pump. All procedures are carried out on a pre-warmed heater mat under a microscope, which allows for the injection and perfusion processes to be monitored. The results suggest that ventricular and atrial myocytes, and fibroblasts can be well isolated from a single adult mouse simultaneously. | |||||
| 書誌情報 |
en : Journal of visualized experiments : JoVE 号 171, 発行日 2021-05-19 |
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| 出版者 | ||||||
| 出版者 | Journal of Visualized Experiments | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1940-087X | |||||
| PMID | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 34096909 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.3791/61866 | |||||
| 関連名称 | 10.3791/61866 | |||||
| 権利 | ||||||
| 権利情報 | © 2021 JoVE Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal Article | |||||
