{"created":"2023-06-19T10:43:39.918698+00:00","id":3929,"links":{},"metadata":{"_buckets":{"deposit":"8af83812-c7fe-46d4-ba70-942b3cc16a36"},"_deposit":{"created_by":11,"id":"3929","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3929"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003929","sets":["30:96:97"]},"author_link":["7406","7489","3686","1532"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"171","bibliographic_titles":[{},{"bibliographic_title":"Journal of visualized experiments : JoVE","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"尾松, 万里子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"福永, 諒"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松浦, 博"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In basic research using mouse heart, isolating viable individual cardiomyocytes is a crucial technical step to overcome. Traditionally, isolating cardiomyocytes from rabbits, guinea pigs or rats has been performed via retrograde perfusion of the heart with enzymes using a Langendorff apparatus. However, a high degree of skill is required when this method is used with a small mouse heart. An antegrade perfusion method that does not use a Langendorff apparatus was recently reported for the isolation of mouse cardiomyocytes. We herein report a complete protocol for the improved antegrade perfusion of the excised heart to isolate individual heart cells from adult mice (8 - 108 weeks old). Antegrade perfusion is performed by injecting perfusate near the apex of the left ventricle of the excised heart, the aorta of which was clamped, using an infusion pump. All procedures are carried out on a pre-warmed heater mat under a microscope, which allows for the injection and perfusion processes to be monitored. The results suggest that ventricular and atrial myocytes, and fibroblasts can be well isolated from a single adult mouse simultaneously.","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Journal of Visualized Experiments"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"34096909","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.3791/61866"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3791/61866","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 JoVE Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1940-087X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OMATSU-KANBE, Mariko"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"FUKUNAGA, Ryo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MI, Xinya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MATSUURA, Hiroshi"}],"nameIdentifiers":[{},{},{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice."}]},"item_type_id":"4","owner":"11","path":["97"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-06-22"},"publish_date":"2021-06-22","publish_status":"0","recid":"3929","relation_version_is_last":true,"title":["An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T11:27:20.376974+00:00"}