WEKO3
-
RootNode
アイテム
Open-channel blocking action of volatile anaesthetics desflurane and sevoflurane on human voltage-gated K v 1.5 channel.
http://hdl.handle.net/10422/00012759
http://hdl.handle.net/10422/000127597f4af8d1-bcf2-4556-a1f3-94f867670257
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-07-14 | |||||
| タイトル | ||||||
| タイトル | Open-channel blocking action of volatile anaesthetics desflurane and sevoflurane on human voltage-gated K v 1.5 channel. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
FUKUSHIMA, Yutaka
× FUKUSHIMA, Yutaka× KOJIMA, Akiko× MI, Xinya× DING, Wei-guang× KITAGAWA, Hirotoshi× MATSUURA, Hiroshi |
|||||
| 著者別名 |
福島, 豊
× 福島, 豊× 小嶋, 亜希子× 北川, 裕利× 松浦, 博 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background and purpose: Volatile anaesthetics have been shown to differentially modulate mammalian Shaker-related voltage-gated potassium (Kv 1.x) channels. This study was designed to investigate molecular and cellular mechanisms underlying the modulatory effects of desflurane or sevoflurane on human Kv 1.5 (hKv 1.5) channels. |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Experimental approach: Thirteen single-point mutations were constructed within pore domain of hKv 1.5 channel using site-directed mutagenesis. The effects of desflurane or sevoflurane on heterologously expressed wild-type and mutant hKv 1.5 channels were examined by whole-cell patch-clamp technique. A computer simulation was conducted to predict the docking pose of desflurane or sevoflurane within hKv 1.5 channel. |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Key results: Both desflurane and sevoflurane increased hKv 1.5 current at mild depolarizations but decreased it at strong depolarizations, indicating that these anaesthetics produce both stimulatory and inhibitory actions on hKv 1.5 channels. The inhibitory effect of desflurane or sevoflurane on hKv 1.5 channels arose primarily from its open-channel blocking action. The inhibitory action of desflurane or sevoflurane on hKv 1.5 channels was significantly attenuated in T480A, V505A, and I508A mutant channels, compared with wild-type channel. Computational docking simulation predicted that desflurane or sevoflurane resides within the inner cavity of channel pore and has contact with Thr479, Thr480, Val505, and Ile508. |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Conclusion and implications: Desflurane and sevoflurane exert an open-channel blocking action on hKv 1.5 channels by functionally interacting with specific amino acids located within the channel pore. This study thus identifies a novel molecular basis mediating inhibitory modulation of hKv 1.5 channels by desflurane and sevoflurane. |
|||||
| 書誌情報 |
en : British journal of pharmacology 発行日 2020-05-20 |
|||||
| 出版者 | ||||||
| 出版者 | Wiley | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1476-5381 | |||||
| PMID | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 32436224 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/bph.15105 | |||||
| 関連名称 | 10.1111/bph.15105 | |||||
| 権利 | ||||||
| 権利情報 | © 2020 The British Pharmacological Society. | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal Article | |||||
