{"created":"2023-06-19T10:43:25.067255+00:00","id":3641,"links":{},"metadata":{"_buckets":{"deposit":"54825f17-231e-459f-b01c-15a9711d6499"},"_deposit":{"created_by":11,"id":"3641","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3641"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003641","sets":["30:253:254","30:96:97"]},"author_link":["3686","1988","3687","7489","742","8002"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-05-20","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{},{"bibliographic_title":"British journal of pharmacology","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"福島, 豊"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"小嶋, 亜希子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"北川, 裕利"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"松浦, 博"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background and purpose:\nVolatile anaesthetics have been shown to differentially modulate mammalian Shaker-related voltage-gated potassium (Kv 1.x) channels. This study was designed to investigate molecular and cellular mechanisms underlying the modulatory effects of desflurane or sevoflurane on human Kv 1.5 (hKv 1.5) channels. ","subitem_description_type":"Abstract"},{"subitem_description":"Experimental approach:\nThirteen single-point mutations were constructed within pore domain of hKv 1.5 channel using site-directed mutagenesis. The effects of desflurane or sevoflurane on heterologously expressed wild-type and mutant hKv 1.5 channels were examined by whole-cell patch-clamp technique. A computer simulation was conducted to predict the docking pose of desflurane or sevoflurane within hKv 1.5 channel. ","subitem_description_type":"Abstract"},{"subitem_description":"Key results:\nBoth desflurane and sevoflurane increased hKv 1.5 current at mild depolarizations but decreased it at strong depolarizations, indicating that these anaesthetics produce both stimulatory and inhibitory actions on hKv 1.5 channels. The inhibitory effect of desflurane or sevoflurane on hKv 1.5 channels arose primarily from its open-channel blocking action. The inhibitory action of desflurane or sevoflurane on hKv 1.5 channels was significantly attenuated in T480A, V505A, and I508A mutant channels, compared with wild-type channel. Computational docking simulation predicted that desflurane or sevoflurane resides within the inner cavity of channel pore and has contact with Thr479, Thr480, Val505, and Ile508. ","subitem_description_type":"Abstract"},{"subitem_description":"Conclusion and implications:\nDesflurane and sevoflurane exert an open-channel blocking action on hKv 1.5 channels by functionally interacting with specific amino acids located within the channel pore. This study thus identifies a novel molecular basis mediating inhibitory modulation of hKv 1.5 channels by desflurane and sevoflurane. ","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"32436224","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1111/bph.15105"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/bph.15105","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2020 The British Pharmacological Society."}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1476-5381","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"FUKUSHIMA, Yutaka"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"KOJIMA, Akiko"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"MI, Xinya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"DING, Wei-guang"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"KITAGAWA, Hirotoshi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"MATSUURA, Hiroshi"}],"nameIdentifiers":[{},{},{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Open-channel blocking action of volatile anaesthetics desflurane and sevoflurane on human voltage-gated K v 1.5 channel.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Open-channel blocking action of volatile anaesthetics desflurane and sevoflurane on human voltage-gated K v 1.5 channel."}]},"item_type_id":"4","owner":"11","path":["97","254"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-07-14"},"publish_date":"2020-07-14","publish_status":"0","recid":"3641","relation_version_is_last":true,"title":["Open-channel blocking action of volatile anaesthetics desflurane and sevoflurane on human voltage-gated K v 1.5 channel."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T12:15:47.194062+00:00"}