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Protocols for Enzymatic Fluorometric Assays to Quantify Phospholipid Classes.
http://hdl.handle.net/10422/00012620
http://hdl.handle.net/10422/0001262007f26689-fe75-4a4b-bdc2-be8a06db088e
名前 / ファイル | ライセンス | アクション |
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ijms21031032 (2.0 MB)
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© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-02-25 | |||||
タイトル | ||||||
タイトル | Protocols for Enzymatic Fluorometric Assays to Quantify Phospholipid Classes. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cardiolipin | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | enzymatic fluorometric measurement | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phosphatidic acid | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phosphatidylcholine | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phosphatidylethanolamine | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phosphatidylglycerol | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phosphatidylinositol | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phosphatidylserine | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | sphingomyelin | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
森田, 真也
× 森田, 真也× TSUJI, Tokuji× TERADA, Tomohiro |
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著者別名 |
森田, 真也
× 森田, 真也× 辻, 徳治× 寺田, 智祐 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Phospholipids, consisting of a hydrophilic head group and two hydrophobic acyl chains, are essential for the structures of cell membranes, plasma lipoproteins, biliary mixed micelles, pulmonary surfactants, and extracellular vesicles. Beyond their structural roles, phospholipids have important roles in numerous biological processes. Thus, abnormalities in the metabolism and transport of phospholipids are involved in many diseases, including dyslipidemia, atherosclerosis, cholestasis, drug-induced liver injury, neurological diseases, autoimmune diseases, respiratory diseases, myopathies, and cancers. To further clarify the physiological, pathological, and molecular mechanisms and to identify disease biomarkers, we have recently developed enzymatic fluorometric assays for quantifying all major phospholipid classes, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidic acid, phosphatidylinositol, phosphatidylglycerol + cardiolipin, and sphingomyelin. These assays are specific, sensitive, simple, and high-throughput, and will be applicable to cells, intracellular organelles, tissues, fluids, lipoproteins, and extracellular vesicles. In this review, we present the detailed protocols for the enzymatic fluorometric measurements of phospholipid classes in cultured cells. | |||||
書誌情報 |
en : International journal of molecular sciences 巻 21, 号 3, p. E1032, 発行日 2020-02-04 |
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出版者 | ||||||
出版者 | MDPI | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1422-0067 | |||||
PMID | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 32033167 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3390/ijms21031032 | |||||
関連名称 | 10.3390/ijms21031032 | |||||
権利 | ||||||
権利情報 | © 2020 by the authors. | |||||
権利 | ||||||
権利情報 | Licensee MDPI, Basel, Switzerland. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal Article |