{"created":"2023-06-19T10:43:17.273666+00:00","id":3497,"links":{},"metadata":{"_buckets":{"deposit":"3e887882-863e-441a-87be-a2bd9951a264"},"_deposit":{"created_by":11,"id":"3497","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3497"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003497","sets":["77:78:79"]},"author_link":["675","4010","7756"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-02-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageStart":"E1032","bibliographicVolumeNumber":"21","bibliographic_titles":[{},{"bibliographic_title":"International journal of molecular sciences","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森田, 真也"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"辻, 徳治"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"寺田, 智祐"}],"nameIdentifiers":[{},{}]}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Phospholipids, consisting of a hydrophilic head group and two hydrophobic acyl chains, are essential for the structures of cell membranes, plasma lipoproteins, biliary mixed micelles, pulmonary surfactants, and extracellular vesicles. Beyond their structural roles, phospholipids have important roles in numerous biological processes. Thus, abnormalities in the metabolism and transport of phospholipids are involved in many diseases, including dyslipidemia, atherosclerosis, cholestasis, drug-induced liver injury, neurological diseases, autoimmune diseases, respiratory diseases, myopathies, and cancers. To further clarify the physiological, pathological, and molecular mechanisms and to identify disease biomarkers, we have recently developed enzymatic fluorometric assays for quantifying all major phospholipid classes, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidic acid, phosphatidylinositol, phosphatidylglycerol + cardiolipin, and sphingomyelin. These assays are specific, sensitive, simple, and high-throughput, and will be applicable to cells, intracellular organelles, tissues, fluids, lipoproteins, and extracellular vesicles. In this review, we present the detailed protocols for the enzymatic fluorometric measurements of phospholipid classes in cultured cells.","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"MDPI"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"32033167","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.3390/ijms21031032"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3390/ijms21031032","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2020 by the authors."},{"subitem_rights":"Licensee MDPI, Basel, Switzerland."}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1422-0067","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森田, 真也"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"TSUJI, Tokuji"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"TERADA, Tomohiro"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-25"}],"displaytype":"detail","filename":"ijms21031032.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","license_note":"© 2020 by the authors. Licensee MDPI, Basel, Switzerland.\nThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution \n(CC BY) license (http://creativecommons.org/licenses/by/4.0/).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ijms21031032","url":"https://shiga-med.repo.nii.ac.jp/record/3497/files/ijms21031032.pdf"},"version_id":"e650b5fe-5feb-46db-92ba-6e8aaccbb409"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cardiolipin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"enzymatic fluorometric measurement","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphatidic acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphatidylcholine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphatidylethanolamine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphatidylglycerol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphatidylinositol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphatidylserine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sphingomyelin","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Protocols for Enzymatic Fluorometric Assays to Quantify Phospholipid Classes.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Protocols for Enzymatic Fluorometric Assays to Quantify Phospholipid Classes."}]},"item_type_id":"4","owner":"11","path":["79"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-02-25"},"publish_date":"2020-02-25","publish_status":"0","recid":"3497","relation_version_is_last":true,"title":["Protocols for Enzymatic Fluorometric Assays to Quantify Phospholipid Classes."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T12:16:08.026687+00:00"}