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Comprehensive evaluation of ubiquitous promoters suitable for the generation of transgenic cynomolgus monkeys†.
http://hdl.handle.net/10422/00012485
http://hdl.handle.net/10422/00012485b26621c4-1f2a-48e3-9939-345b314d136f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ioz040 (852.5 kB)
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© 2019, Oxford University Press
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-03-28 | |||||
| タイトル | ||||||
| タイトル | Comprehensive evaluation of ubiquitous promoters suitable for the generation of transgenic cynomolgus monkeys†. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Blastocyst | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Differentiation | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Embryonic stem cells | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Intracytoplasmic sperm injection (ICSI) | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Primates | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Zygote | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Seita, Yasunari
× Seita, Yasunari× Tsukiyama, Tomoyuki× Azami, Takuya× Kobayashi, Kenichi× IWATANI, Chizuru× TSUCHIYA, Hideaki× Nakaya, Masataka× Tanabe, Hideyuki× Hitoshi, Seiji× Miyoshi, Hiroyuki× Nakamura, Shin-ichiro× Kawauchi, Akihiro× Ema, Masatsugu |
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| 著者別名 |
清田, 弥寿成
× 清田, 弥寿成× 築山, 智之× 浅見, 拓哉× 小林, 憲市× 岩谷, 千鶴× 土屋, 英明× 中家, 雅隆× 等, 誠司× 中村, 紳一郎× 河内, 明宏× 依馬, 正次 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Nonhuman primates (NHPs) are considered to be the most valuable models for human transgenic (Tg) research into disease, because human pathology is more closely recapitulated in NHPs than rodents. Previous studies have reported the generation of Tg NHPs that ubiquitously overexpress a transgene using various promoters, but it is not yet clear which promoter is most suitable for the generation of NHPs overexpressing a transgene ubiquitously and persistently in various tissues. To clarify this issue, we evaluated four putative ubiquitous promoters, cytomegalovirus (CMV) immediate-early enhancer and chicken beta-actin (CAG), Elongation factor 1α (EF1α), Ubiquitin C (UbC), and CMV, using an in vitro differentiation system of cynomolgus monkey embryonic stem cells (ESCs). While the EF1α promoter drove Tg expression more strongly than the other promoters in undifferentiated pluripotent ESCs, the CAG promoter was more effective in differentiated cells such as embryoid bodies and ESC-derived neurons. When the CAG and EF1α promoters were used to generate green fluorescent protein (GFP)-expressing Tg monkeys, the CAG promoter drove GFP expression in skin and hematopoietic tissues more strongly than in ΕF1α-GFP Tg monkeys. Notably, the EF1α promoter underwent more silencing in both ESCs and Tg monkeys. Thus, the CAG promoter appears to be the most suitable for ubiquitous and stable expression of transgenes in the differentiated tissues of Tg cynomolgus monkeys and appropriate for the establishment of human disease models. | |||||
| 書誌情報 |
Biology of Reproduction 発行日 2019-03-14 |
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| 出版者 | ||||||
| 出版者 | Society for the Study of Reproduction | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1529-7268 | |||||
| PMID | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 30869744 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1093/biolre/ioz040 | |||||
| 権利 | ||||||
| 権利情報 | © The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal Article | |||||
