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Gene Therapy for Neuropathic Pain through siRNA-IRF5 Gene Delivery with Homing Peptides to Microglia.
http://hdl.handle.net/10422/00012422
http://hdl.handle.net/10422/0001242225a6610c-5220-498a-b10a-e33ed0299857
| 名前 / ファイル | ライセンス | アクション |
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j.omtn.2018.02.007 (3.6 MB)
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j.omtn.2018.02.007extended (4.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-06-27 | |||||
| タイトル | ||||||
| タイトル | Gene Therapy for Neuropathic Pain through siRNA-IRF5 Gene Delivery with Homing Peptides to Microglia. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | homing peptides | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | gene delivery | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | microglia | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | interferon regulatory factor 5 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | IRF | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | astrocyte | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
寺島, 智也
× 寺島, 智也× Ogawa, Nobuhiro× Nakase, Yuki× Sato, Toshiyuki× Katagi, Miwako× Okano, Junko× MAEGAWA, Hiroshi× KOJIMA, Hideto |
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| 著者別名 |
寺島, 智也
× 寺島, 智也× 小川, 暢弘× 樫, 美和子× 岡野, 純子× 前川, 聡× 小島, 秀人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Astrocyte- and microglia-targeting peptides were identified and isolated using phage display technology. A series of procedures, including three cycles of both in vivo and in vitro biopanning, was performed separately in astrocytes and in M1 or M2 microglia, yielding 50-58 phage plaques in each cell type. Analyses of the sequences of this collection identified one candidate homing peptide targeting astrocytes (AS1[C-LNSSQPS-C]) and two candidate homing peptides targeting microglia (MG1[C-HHSSSAR-C] and MG2[C-NTGSPYE-C]). To determine peptide specificity for the target cell in vitro, each peptide was synthesized and introduced into the primary cultures of astrocytes or microglia. Those peptides could bind to the target cells and be selectively taken up by the corresponding cell, namely, astrocytes, M1 microglia, or M2 microglia. To confirm cell-specific gene delivery to M1 microglia, the complexes between peptide MG1 and siRNA-interferon regulatory factor 5 were prepared and intrathecally injected into a mouse model of neuropathic pain. The complexes successfully suppressed hyperalgesia with high efficiency in this neuropathic pain model. Here, we describe a novel gene therapy for the treatment neuropathic pain, which has a high potential to be of clinical relevance. This strategy will ensure the targeted delivery of therapeutic genes while minimizing side effects to non-target tissues or cells. |
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| 書誌情報 |
Molecular therapy. Nucleic acids 巻 11, p. 203-215, 発行日 2018-03-28 |
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| 出版者 | ||||||
| 出版者 | Elsevier (Cell Press) | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2162-2531 | |||||
| PMID | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 29858055 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.omtn.2018.02.007 | |||||
| 権利 | ||||||
| 権利情報 | © 2018 The Author(s). | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal Article | |||||
