{"created":"2023-06-19T10:43:07.829875+00:00","id":3284,"links":{},"metadata":{"_buckets":{"deposit":"ce113e27-714b-42d1-acd2-49d7b79669f9"},"_deposit":{"created_by":11,"id":"3284","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3284"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003284","sets":["30:202:203"]},"author_link":["7211","534","552","7332","7333","538","531","550"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03-28","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"215","bibliographicPageStart":"203","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"Molecular therapy. Nucleic acids"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"寺島, 智也"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"小川, 暢弘"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"樫, 美和子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"岡野, 純子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"前川, 聡"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"小島, 秀人"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Astrocyte- and microglia-targeting peptides were identified and isolated using phage \ndisplay technology. A series of procedures, including three cycles of both in vivo and \nin vitro biopanning, was performed separately in astrocytes and in M1 or M2 microglia,\nyielding 50-58 phage plaques in each cell type. Analyses of the sequences of this \ncollection identified one candidate homing peptide targeting astrocytes (AS1[C-LNSSQPS-C]) and two candidate homing peptides targeting microglia (MG1[C-HHSSSAR-C] and MG2[C-NTGSPYE-C]). To determine peptide specificity for the target cell in vitro, \neach peptide was synthesized and introduced into the primary cultures of astrocytes or\n microglia. Those peptides could bind to the target cells and be selectively taken up by \nthe corresponding cell, namely, astrocytes, M1 microglia, or M2 microglia. To confirm \ncell-specific gene delivery to M1 microglia, the complexes between peptide MG1 and \nsiRNA-interferon regulatory factor 5 were prepared and intrathecally injected into a \nmouse model of neuropathic pain. The complexes successfully suppressed hyperalgesia \nwith high efficiency in this neuropathic pain model. Here, we describe a novel gene \ntherapy for the treatment neuropathic pain, which has a high potential to be of clinical \nrelevance. This strategy will ensure the targeted delivery of therapeutic genes while \nminimizing side effects to non-target tissues or cells.","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier (Cell Press)"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"29858055","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.omtn.2018.02.007","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2018 The Author(s)."}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2162-2531","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"寺島, 智也"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Ogawa, Nobuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakase, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Toshiyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Katagi, Miwako"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Okano, Junko"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"MAEGAWA, Hiroshi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"KOJIMA, Hideto"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-27"}],"displaytype":"detail","filename":"j.omtn.2018.02.007.pdf","filesize":[{"value":"3.6 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"j.omtn.2018.02.007","url":"https://shiga-med.repo.nii.ac.jp/record/3284/files/j.omtn.2018.02.007.pdf"},"version_id":"e35a9107-870c-49ce-aaf5-b4d553f51f26"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-06-27"}],"displaytype":"detail","filename":"j.omtn.2018.02.007extended.pdf","filesize":[{"value":"4.1 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"j.omtn.2018.02.007extended","url":"https://shiga-med.repo.nii.ac.jp/record/3284/files/j.omtn.2018.02.007extended.pdf"},"version_id":"994767f5-55d5-4f4f-aebe-8e4e69d5f82f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"homing peptides","subitem_subject_scheme":"Other"},{"subitem_subject":"gene delivery","subitem_subject_scheme":"Other"},{"subitem_subject":"microglia","subitem_subject_scheme":"Other"},{"subitem_subject":"interferon regulatory factor 5","subitem_subject_scheme":"Other"},{"subitem_subject":"IRF","subitem_subject_scheme":"Other"},{"subitem_subject":"astrocyte","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Gene Therapy for Neuropathic Pain through siRNA-IRF5 Gene Delivery with Homing Peptides to Microglia.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Gene Therapy for Neuropathic Pain through siRNA-IRF5 Gene Delivery with Homing Peptides to Microglia."}]},"item_type_id":"4","owner":"11","path":["203"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-06-27"},"publish_date":"2018-06-27","publish_status":"0","recid":"3284","relation_version_is_last":true,"title":["Gene Therapy for Neuropathic Pain through siRNA-IRF5 Gene Delivery with Homing Peptides to Microglia."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T11:39:45.221376+00:00"}