@article{oai:shiga-med.repo.nii.ac.jp:00000093,
 author = {SU, Nan-Chang and YAMAMOTO, Gaku and KIMURA, Yusuke and YOSHITAKE, Kazusada},
 journal = {滋賀医科大学雑誌},
 month = {Feb},
 note = {We tested the nacre of the freshwater mollusk Hyriopsis schlegeli for its ability to induce the osteogenesisin the present study. Powdered EDTA-decalcified (0.3-1mm) and undecalcified nacre (300-425μm)were cultured with mouse osteoblasts (MC3T3-E1) for four weeks. Three experimental groups were comparedin the present study. Medium for the culture was a combination of alpha-modified Eagle's medium, 10 %fetal bovine serum, and 1 % L-glutamine. The group A (n=10) applied undecalcified nacreous powder alone,whereas the group B (n=10) applied only decalcified nacreous powder. In group A and B, one grain of nacreouspowder was placed in each dish. The group C (n=20) applied one decalcified and one undecalcifiednacreous grains in each dish. These two grains were placed at a distance of less than 1mm. The sampleswere stained with alizarin red S, von Kossa, van Gieson, Mallory, and alcian blue at the end of the 4-weekculture. The results showed that the newly formed meshed tissue around the decalcified nacre was lessprominent than that around the undecalcified nacre. The meshed tissue around the decalcified nacre waspositively stained with van Gieson and Mallory, which are considered to be specific for collagen. Themeshed tissue around the undecalcified nacre was presumably osteoid tissue because it was positivelystained with all stains except alcian blue. Thus, the undecalcified nacre of freshwater mollusk Hyriopsisschlegeli may induce osteogenesis, while the decalcified nacre is less potent to induce calcified osteoid.},
 pages = {63--71},
 title = {Effects of decalcified and undecalcified nacre of freshwater mollusk Hyriopsis schlegeli on mouse osteoblasts (MC3T3-E1) to induce osteoid in vitro},
 volume = {17},
 year = {2002}
}