{"created":"2023-06-19T10:40:56.718813+00:00","id":507,"links":{},"metadata":{"_buckets":{"deposit":"77fc35c5-84e6-4506-95fb-4a7b03cf6a97"},"_deposit":{"created_by":3,"id":"507","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"507"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00000507","sets":["6:9:177"]},"author_link":["130"],"item_3_alternative_title_18":{"attribute_name":"その他の言語のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Quantitative PCR to evaluate small amounts of mRNA"}]},"item_3_alternative_title_19":{"attribute_name":"タイトル（ヨミ）","attribute_value_mlt":[{"subitem_alternative_title":"ツウジョウ ノ PCR ソウチ ヲ モチイタ ビリョウ mRNA テイリョウ"}]},"item_3_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"38","bibliographicPageStart":"35","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"滋賀医科大学基礎学研究"}]}]},"item_3_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Departmental Bulletin Paper","subitem_description_type":"Other"}]},"item_3_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本研究はヒトの末梢血中に含まれるアポトーシス関連タンパクであるBcl-2のmRNAの微量定量を通常のPCR装置をつかって行うことを目的とした。3倍づつ濃度を変化させた4種類の既知濃度の競合鋳型(competitor)を含む4種類の試験管を用意し、それぞれの試験管内に同量のサンプルを加えて反応(PCR)を行った。サンプルの添加量を変化させ、PCR後に4本の試験管で増幅されたサンプルの合計とcompetitorの合計が等しくなるようにした。この条件下でサンプルと同量がPCRで増幅されたcompetitorの濃度を、グラフを使って求めることにより、微量のmRNAの値を再現性よく測定することができた。この方法では従来の廉価なPCR装置によって微量なmRNAを定量することができるだけでなく、サンプル中の不純物により定量目的でつくられたPCR装置よって定量がうまく行かないサンプルも、そのmRNA量の測定が可能であった。","subitem_description_type":"Other"}]},"item_3_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"滋賀医科大学"}]},"item_3_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10217414","subitem_source_identifier_type":"NCID"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"佐藤, 浩"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-16"}],"displaytype":"detail","filename":"kiso12p035.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kiso12p035.pdf","url":"https://shiga-med.repo.nii.ac.jp/record/507/files/kiso12p035.pdf"},"version_id":"09d10e8f-7e3b-4cb0-84ea-7ad2e36b5169"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"通常のPCR装置を用いた微量mRNA定量","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"通常のPCR装置を用いた微量mRNA定量"}]},"item_type_id":"3","owner":"3","path":["177"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-08-24"},"publish_date":"2012-08-24","publish_status":"0","recid":"507","relation_version_is_last":true,"title":["通常のPCR装置を用いた微量mRNA定量"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-19T12:29:35.613972+00:00"}