@article{oai:shiga-med.repo.nii.ac.jp:00004300,
 author = {三宅, 亨 and 前川, 毅 and 森, 治樹 and 安川, 大貴 and 大野, 将司 and 西田, 淳史 and 安藤, 朗 and 谷, 眞至 and HEXUN  , Zhang and MIYAKE, Toru and MAEKAWA, Takeru and MORI, Haruki and YASUKAWA, Daiki and OHNO, Masashi and NISHIDA, Atsushi and ANDOH, Akira and TANI, Masaji},
 journal = {Cancer Immunology, Immunotherapy},
 month = {Jul},
 note = {Introduction:
The tumor microenvironment (TME) in colorectal cancer (CRC) includes the gut microbiome, immune cells, angiogenic factors, and fibroblasts and plays a major role in cancer progression. The Immunoscore (IS) is based on tumor infiltration by immune cells that are known prognostic biomarkers for CRC. However, the interrelation between the IS, microbiome, and other TME factors in human CRC remains unclear., Patients and methods:
A cohort of 94 patients with CRC was examined at the Shiga University of Medical Science Hospital in Japan. The expression levels of CD3, CD8, CD31, and alpha-smooth muscle actin (α-SMA) in the primary tumor were evaluated by immunohistochemistry. The IS was calculated based on the results of the CD3 and CD8 staining assays. Microbiomes in patients with CRC were examined by amplicon sequencing., Results:
The expression levels of α-SMA and tumor-infiltrating lymphocytes in patients with CRC were negatively correlated (P = 0.006). A high IS was associated with high abundance of Lachnospiraceae in the microbiomes of patients with CRC., Conclusion:
Lymphocyte infiltration into the primary tumor was marked by reduced density of cancer-associated fibroblasts and enrichment of the Lachnospiraceae family in the gut microbiome, which may influence CRC progression., Journal Article},
 title = {High abundance of Lachnospiraceae in the human gut microbiome is related to high immunoscores in advanced colorectal cancer.},
 year = {2022}
}