{"created":"2023-06-19T10:43:51.793783+00:00","id":4207,"links":{},"metadata":{"_buckets":{"deposit":"ac0d9a33-b4fc-43e1-82cc-be176ce29b7c"},"_deposit":{"created_by":11,"id":"4207","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"4207"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00004207","sets":["30:198:199","30:202:203"]},"author_link":["534","550","7935","531","1956"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-04-04","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{},{"bibliographic_title":"Cytotherapy","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小橋, 修平"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"寺島, 智也"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"樫, 美和子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"漆谷, 真"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"小島, 秀人"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background aims:\nAmyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disease. Neuroinflammation in the spinal cord plays a pivotal role in the pathogenesis of ALS, and microglia are involved in neuroinflammation. Microglia mainly have two opposite phenotypes involving cytotoxic and neuroprotective properties, and neuroprotective microglia are expected to be a novel application for the treatment of ALS. Therefore, to establish a clinically applicable therapeutic method using neuroprotective microglia, the authors investigated the effect of inducing neuroprotective microglia-like cells from bone marrow for transplantation into ALS model mice. ","subitem_description_type":"Abstract"},{"subitem_description":"Methods:\nBone marrow-derived mononuclear cells were isolated from green fluorescent protein mice and cultured using different protocols of cytokine treatment with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. Cells with a high potency of proliferation and differentiation into microglia were evaluated by gene analysis, flow cytometry and direct neuroprotective effects in vitro. These cells were named bone marrow-derived inducible microglia-like (BM-iMG) cells and transplanted into the spinal cords of ALS model mice, and behavioral tests, immunohistochemistry and gene expression profiling were performed. ","subitem_description_type":"Abstract"},{"subitem_description":"Results:\nThree-day GM-CSF and 4-day GM-CSF + IL-4 stimulations were most effective in inducing BM-iMG cells from the bone marrow. Transplantation of BM-iMG cells improved motor function, prolonged survival and suppressed neuronal cell death, astrogliosis and microgliosis in the spinal cords of ALS mice. Moreover, neuroprotective genes such as Arg1 and Mrc1 were upregulated, whereas pro-inflammatory genes such as Nos2 and Il6 were downregulated. ","subitem_description_type":"Abstract"},{"subitem_description":"Conclusions:\nIntraspinal transplantation of BM-iMG cells demonstrated therapeutic effects in a mouse model of ALS. Further studies and clinical applications in patients with ALS are expected in the future. ","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"35393241","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.jcyt.2022.02.001"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jcyt.2022.02.001","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2022 International Society for Cell & Gene Therapy. Published by Elsevier Inc. All rights reserved. "}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1477-2566","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KOBASHI, Shuhei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TERASHIMA, Tomoya"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"KATAGI, Miwako"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"URUSHITANI, Makoto"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"KOJIMA, Hideto"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-05-10"}],"displaytype":"detail","filename":"j.jcyt.2022.02.001.pdf","filesize":[{"value":"5.3 MB"}],"format":"application/pdf","license_note":"This is an open access articleunder the CC BY-NC-ND license \n(http://creativecommons.org/licenses/by-nc-nd/4.0/)","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"j.jcyt.2022.02.001","url":"https://shiga-med.repo.nii.ac.jp/record/4207/files/j.jcyt.2022.02.001.pdf"},"version_id":"377cdd5f-4269-422d-a7e4-8408b365f3d0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"amyotrophic lateral sclerosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"BM-iMG cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bone marrow-derived cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GM-CSF","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"IL-4","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"neuroprotective microglia","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Bone marrow-derived inducible microglia-like cells ameliorate motor function and survival in a mouse model of amyotrophic lateral sclerosis.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bone marrow-derived inducible microglia-like cells ameliorate motor function and survival in a mouse model of amyotrophic lateral sclerosis."}]},"item_type_id":"4","owner":"11","path":["199","203"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-10"},"publish_date":"2022-05-10","publish_status":"0","recid":"4207","relation_version_is_last":true,"title":["Bone marrow-derived inducible microglia-like cells ameliorate motor function and survival in a mouse model of amyotrophic lateral sclerosis."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T11:15:56.158162+00:00"}