{"created":"2023-06-19T10:43:46.942502+00:00","id":4095,"links":{},"metadata":{"_buckets":{"deposit":"27a69cef-5942-4720-9ace-5b6b3b58e770"},"_deposit":{"created_by":11,"id":"4095","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"4095"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00004095","sets":["19:34:35"]},"author_link":["8818","7552","8820","7626","1957","7627","8819"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-12","bibliographicIssueDateType":"Issued"},"bibliographicVolumeNumber":"28","bibliographic_titles":[{},{"bibliographic_title":"Biochemistry and Biophysics Reports","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"柳沢, 大治郎 "}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"遠山, 育夫"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"One of the neuropathological hallmarks of Alzheimer's disease (AD)-causing neurodegeneration and consequent memory deterioration, and eventually, cognitive decline-is amyloid-β (Aβ) aggregation forming amyloid plaques. Our previous study showed the potential of a tocotrienol-rich fraction-a mixture of naturally occurring of vitamin E analogs-to inhibit Aβ aggregation and restore cognitive function in an AD mouse model. The current study examined the effect of three vitamin E analogs-α-tocopherol (α-TOC), α-tocotrienol (α-T3), and γ-tocotrienol (γ-T3)-on Aβ aggregation, disaggregation, and oligomerization in vitro. Thioflavin T (ThT) assay showed α-T3 reduced Aβ aggregation at 10 μM concentration. Furthermore, both α-T3 and γ-T3 demonstrated Aβ disaggregation, as shown by the reduction of ThT fluorescence. However, α-TOC showed no significant effect. We confirmed the results for ThT assays with scanning electron microscopy imaging. Further investigation in photo-induced cross-linking of unmodified protein assay indicated a reduction in Aβ oligomerization by γ-T3. The present study thus revealed the individual effect of each tocotrienol analog in reducing Aβ aggregation and oligomerization as well as disaggregating preformed fibrils. ","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"34541343","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.bbrep.2021.101131"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrep.2021.101131","subitem_relation_type_select":"DOI"}}]},"item_4_relation_13":{"attribute_name":"PMCID","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"PMC8435913"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc8435913/","subitem_relation_type_select":"URI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 The Authors. "}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2405-5808","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"IBRAHIM, Nor Faeizah"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"HAMEZAH, Hamizah Shahirah Binti"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YANAGISAWA, Daijiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"TSUJI, Mayumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KIUCHI, Yuji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ONO, Kenjiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TOOYAMA, Ikuo"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-24"}],"displaytype":"detail","filename":"j.bbrep.2021.101131.pdf","filesize":[{"value":"5.3 MB"}],"format":"application/pdf","license_note":"This is an open access article under the CC BY-NC-ND license\n(http://creativecommons.org/licenses/by-nc-nd/4.0/).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"j.bbrep.2021.101131","url":"https://shiga-med.repo.nii.ac.jp/record/4095/files/j.bbrep.2021.101131.pdf"},"version_id":"3cfb5a71-6fd6-40db-9d74-b0f669105947"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Amyloid-β","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tocotrienol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tocopherol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Vitamin E","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Alzheimer's disease","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The effect of α-tocopherol, α- and γ-tocotrienols on amyloid-β aggregation and disaggregation in vitro.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The effect of α-tocopherol, α- and γ-tocotrienols on amyloid-β aggregation and disaggregation in vitro."}]},"item_type_id":"4","owner":"11","path":["35"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-24"},"publish_date":"2022-01-24","publish_status":"0","recid":"4095","relation_version_is_last":true,"title":["The effect of α-tocopherol, α- and γ-tocotrienols on amyloid-β aggregation and disaggregation in vitro."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T11:19:11.762674+00:00"}