{"created":"2023-06-19T10:43:46.377360+00:00","id":4082,"links":{},"metadata":{"_buckets":{"deposit":"2d9d708a-855f-4ad1-b407-8c4233f3ec74"},"_deposit":{"created_by":11,"id":"4082","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"4082"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00004082","sets":["30:191:192","30:273:274","30:40:268"]},"author_link":["3912","1961","7872","7839","8800","1857","7833","8801","1971","8803","7832","7840","8802"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03-17","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"86","bibliographicPageStart":"77","bibliographicVolumeNumber":"24","bibliographic_titles":[{},{"bibliographic_title":"Molecular Therapy - Oncolytics","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"寺田, 晃士"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"近藤, 健太"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"石垣, 宏仁"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"里岡, 大樹"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"平田, 多佳子"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"小笠原, 一誠"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"伊藤, 靖"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"縣, 保年"}],"nameIdentifiers":[{},{}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To develop effective adoptive cell transfer therapy using T cell receptor (TCR)-engineered T cells, it is critical to isolate tumor-reactive TCRs that have potent anti-tumor activity. In humans, tumor-infiltrating lymphocytes (TILs) have been reported to contain CD8+PD-1+ T cells that express tumor-reactive TCRs. Characterization of tumor reactivity of TILs from non-human primate tumors could improve anti-tumor activity of TCR-engineered T cells in preclinical research. In this study, we sought to isolate TCR genes from CD8+PD-1+ T cells among TILs in a cynomolgus macaque model of tumor transplantation in which the tumors were infiltrated with CD8+ T cells and were eventually rejected. We analyzed the repertoire of TCRα and β pairs obtained from single CD8+PD-1+ T cells in TILs and circulating lymphocytes and identified multiple TCR pairs with high frequency, suggesting that T cells expressing these recurrent TCRs were clonally expanded in response to tumor cells. We further showed that the recurrent TCRs exhibited cytotoxic activity to tumor cells in vitro and potent anti-tumor activity in mice transplanted with tumor cells. These results imply that this tumor transplantation macaque model recapitulates key features of human TILs and can serve as a platform toward preclinical studies of non-human primate tumor models.","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Cell Press"}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1016/j.omto.2021.12.003"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.omto.2021.12.003","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©2021 The Authors."}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2372-7705","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TERADA, Koji"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"KONDO, Kenta"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"ISHIGAKI, Hirohito"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"NAGASHIMA, Ayaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SATOOKA, Hiroki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"NAGANO, Seiji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MASUDA, Kyoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KAWAMURA, Teruhisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HIRATA, Takako"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"OGASAWARA, Kazumasa"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"ITOH, Yasushi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"KAWAMOTO, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"AGATA, Yasutoshi"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-01-04"}],"displaytype":"detail","filename":"j.omto.2021.12.003.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","license_note":"This is an open access article under the CC BY-NC-ND license \n(http://creativecommons.org/licenses/by-nc-nd/4.0/).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"j.omto.2021.12.003","url":"https://shiga-med.repo.nii.ac.jp/record/4082/files/j.omto.2021.12.003.pdf"},"version_id":"16674d75-fa47-46f6-82e4-ee02b01c7e47"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"adoptive cell transfer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"T cell receptor-engineered T cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tumor-infiltrating lymphocytes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"non-human primates","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"iPS cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"regenerated T cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cytotoxic T lymphocytes (CTLs)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"macaques","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PD-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"TCR repertoire","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Isolation of TCR genes with tumor-killing activity from tumor-infiltrating and circulating lymphocytes in a tumor rejection cynomolgus macaque model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Isolation of TCR genes with tumor-killing activity from tumor-infiltrating and circulating lymphocytes in a tumor rejection cynomolgus macaque model"}]},"item_type_id":"4","owner":"11","path":["192","268","274"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-01-04"},"publish_date":"2022-01-04","publish_status":"0","recid":"4082","relation_version_is_last":true,"title":["Isolation of TCR genes with tumor-killing activity from tumor-infiltrating and circulating lymphocytes in a tumor rejection cynomolgus macaque model"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T11:19:36.396472+00:00"}