{"created":"2023-06-19T10:43:40.003904+00:00","id":3931,"links":{},"metadata":{"_buckets":{"deposit":"cd30da87-9a43-4531-9a55-9ec4ca2ba4a1"},"_deposit":{"created_by":11,"id":"3931","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3931"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003931","sets":["30:202:203","30:403:404"]},"author_link":["7211","534","531","7358","7208","7179","550"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-06-17","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicVolumeNumber":"11","bibliographic_titles":[{},{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大橋, 夏子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"寺島, 智也"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"樫, 美和子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"中江, 由希"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"岡野, 純子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"鈴木, 義久"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"小島, 秀人"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Amyotrophic lateral sclerosis (ALS) is an intractable neurodegenerative disease. CD68-positive bone marrow (BM)-derived cells (BMDCs) accumulate in the pathological lesion in the SOD1(G93A) ALS mouse model after BM transplantation (BMT). Therefore, we investigated whether BMDCs can be applied as gene carriers for cell-based gene therapy by employing the accumulation of BMDCs. In ALS mice, YFP reporter signals were observed in 12-14% of white blood cells (WBCs) and in the spinal cord via transplantation of BM after lentiviral vector (LV) infection. After confirmation of gene transduction by LV with the CD68 promoter in 4-7% of WBCs and in the spinal cord of ALS mice, BM cells were infected with LVs expressing glutamate transporter (GLT) 1 that protects neurons from glutamate toxicity, driven by the CD68 promoter, which were transplanted into ALS mice. The treated mice showed improvement of motor behaviors and prolonged survival. Additionally, interleukin (IL)-1β was significantly suppressed, and IL-4, arginase 1, and FIZZ were significantly increased in the mice. These results suggested that GLT1 expression by BMDCs improved the spinal cord environment. Therefore, our gene therapy strategy may be applied to treat neurodegenerative diseases such as ALS in which BMDCs accumulate in the pathological lesion by BMT. ","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"34140581","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1038/s41598-021-92285-x"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/s41598-021-92285-x","subitem_relation_type_select":"DOI"}}]},"item_4_relation_13":{"attribute_name":"PMCID","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"PMC8211665"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc8211665/","subitem_relation_type_select":"URI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2021"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"OHASHI, Natsuko"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"TERASHIMA, Tomoya"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"KATAGI, Miwako"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"NAKAE, Yuki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"OKANO, Junko"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"SUZUKI, Yoshihisa"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"KOJIMA, Hideto"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-06-29"}],"displaytype":"detail","filename":"s41598-021-92285-x.pdf","filesize":[{"value":"12.8 MB"}],"format":"application/pdf","license_note":"This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"s41598-021-92285-x","url":"https://shiga-med.repo.nii.ac.jp/record/3931/files/s41598-021-92285-x.pdf"},"version_id":"e50201c2-8594-4e0a-85c3-5adeb48a24bb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Genetic transduction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Neurological disorders","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"GLT1 gene delivery based on bone marrow-derived cells ameliorates motor function and survival in a mouse model of ALS.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"GLT1 gene delivery based on bone marrow-derived cells ameliorates motor function and survival in a mouse model of ALS."}]},"item_type_id":"4","owner":"11","path":["203","404"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-06-29"},"publish_date":"2021-06-29","publish_status":"0","recid":"3931","relation_version_is_last":true,"title":["GLT1 gene delivery based on bone marrow-derived cells ameliorates motor function and survival in a mouse model of ALS."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T11:25:29.976379+00:00"}