{"created":"2023-06-19T10:43:36.183137+00:00","id":3850,"links":{},"metadata":{"_buckets":{"deposit":"35d230e3-0bde-4abc-9b63-0751a3d69c0b"},"_deposit":{"created_by":11,"id":"3850","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3850"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003850","sets":["19:34:35"]},"author_link":["8483","7626","8481","6345","1957","8482","8480"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-04-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"274","bibliographicPageStart":"263","bibliographicVolumeNumber":"5","bibliographic_titles":[{},{"bibliographic_title":"Journal of Alzheimer's Disease Reports","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"柳沢, 大治郎 "}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"加藤, 智子"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"遠山, 育夫"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background:\nEmerging evidence indicates that the misfolded tau protein can propagate aggregates between cells in a prion-like manner. This prion activity has been typically studied in brain extracts of patients with Alzheimer’s disease (AD), but not in the olfactory region that can be a potential biomarker in AD.","subitem_description_type":"Abstract"},{"subitem_description":"Objective:\nTo investigate the prion seeding activity of tau in nasal mucosa tissues using a cell culture model of tau propagation.","subitem_description_type":"Abstract"},{"subitem_description":"Methods:\nBrain and nasal mucosa homogenates were added to HEK293T cells expressing three repeat or four-repeat domains of tau with the L266V, V337M (3RD*VM) and P301L and V377M mutations (4RD*LM) fused to the enhanced green fluorescence protein (EGFP) respectively. We also measured the level of phosphorylated tau (p-tau), total tau (t-tau), and p-tau/t-tau ratio and performed correlation analysis between tau prion activity and the level of tau.","subitem_description_type":"Abstract"},{"subitem_description":"Results:\nWe found that brain and nasal tissue homogenates from patients with AD significantly induced tau aggregation in HEK293T cells either expressing tau 3RD*VM-EGFP or 4RD*LM-EGFP compared with control brain and nasal tissue homogenates. The levels of p-tau and p-tau/t-tau ratio were significantly increased in the brain of patients with AD; however, no significant difference was found in nasal tissue compared with their respective control tissue homogenates.","subitem_description_type":"Abstract"},{"subitem_description":"Conclusion:\nThese results suggest that the nasal tissues contain tau seeds, similar to the brain, albeit without changes in the levels of p-tau and t-tau. Therefore, a cellular bioassay using nasal tissues would have great potential as an AD biomarker because of the usefulness of nasal tissue biopsy.","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"IOS Press"}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.3233/ADR-210298"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3233/adr-210298","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2021 – The authors."}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"25424823","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"PAHRUDIN ARROZI, Aslina"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"YANAGISAWA, Daijiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"KATO, Tomoko"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"AKATSU, Hiroyasu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"HASHIZUME, Yoshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KANEDA, Daita"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TOOYAMA, Ikuo"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-04-21"}],"displaytype":"detail","filename":"ADR-210298.pdf","filesize":[{"value":"1.9 MB"}],"format":"application/pdf","license_note":"This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (CC BY-NC 4.0).","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ADR-210298","url":"https://shiga-med.repo.nii.ac.jp/record/3850/files/ADR-210298.pdf"},"version_id":"ea1d5acc-632a-47db-8494-f39fbabe7360"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Alzheimer’s disease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nasal extracts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tau aggregation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tau propagation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Nasal Extracts from Patients with Alzheimer’s Disease Induce Tau Aggregates in a Cellular Model of Tau Propagation.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Nasal Extracts from Patients with Alzheimer’s Disease Induce Tau Aggregates in a Cellular Model of Tau Propagation."}]},"item_type_id":"4","owner":"11","path":["35"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-04-21"},"publish_date":"2021-04-21","publish_status":"0","recid":"3850","relation_version_is_last":true,"title":["Nasal Extracts from Patients with Alzheimer’s Disease Induce Tau Aggregates in a Cellular Model of Tau Propagation."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T11:36:50.165657+00:00"}