{"created":"2023-06-19T10:43:33.619140+00:00","id":3796,"links":{},"metadata":{"_buckets":{"deposit":"eefc2e05-8248-40b6-8d87-8974f8b9318b"},"_deposit":{"created_by":11,"id":"3796","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3796"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003796","sets":["77:78:79"]},"author_link":["675","8353","4010","7156","8354","7756"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"2731","bibliographicVolumeNumber":"11","bibliographic_titles":[{},{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"辻, 徳治"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"森田, 真也"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"中村, 吉伸"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"池田, 義人"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"寺田, 智祐"}],"nameIdentifiers":[{},{}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The human hepatoblastoma cell line, HepG2, has been used for investigating a wide variety of physiological and pathophysiological processes. However, less information is available about the phospholipid metabolism in HepG2 cells. In the present report, to clarify the relationship between cell growth and phospholipid metabolism in HepG2 cells, we examined the phospholipid class compositions of the cells and their intracellular organelles by using enzymatic fluorometric methods. In HepG2 cells, the ratios of all phospholipid classes, but not the ratio of cholesterol, markedly changed with cell growth. Of note, depending on cell growth, the phosphatidic acid (PA) ratio increased and phosphatidylcholine (PC) ratio decreased in the nuclear membranes, the sphingomyelin (SM) ratio increased in the microsomal membranes, and the phosphatidylethanolamine (PE) ratio increased and the phosphatidylserine (PS) ratio decreased in the mitochondrial membranes. Moreover, the mRNA expression levels of enzymes related to PC, PE, PS, PA, SM and cardiolipin syntheses changed during cell growth. We suggest that the phospholipid class compositions of organellar membranes are tightly regulated by cell growth. These findings provide a basis for future investigations of cancer cell growth and lipid metabolism. ","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"33526799","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1038/s41598-021-81733-3"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/s41598-021-81733-3","subitem_relation_type_select":"DOI"}}]},"item_4_relation_13":{"attribute_name":"PMCID","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"PMC7851136"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7851136/","subitem_relation_type_select":"URI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2021"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TSUJI, Tokuji"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"MORITA, Shin-ya"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"NAKAMURA, Yoshinobu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IKEDA, Yoshito"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"KAMBE, Taiho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TERADA, Tomohiro"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-02-10"}],"displaytype":"detail","filename":"s41598-021-81733-3.pdf","filesize":[{"value":"4.6 MB"}],"format":"application/pdf","license_note":"This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"s41598-021-81733-3","url":"https://shiga-med.repo.nii.ac.jp/record/3796/files/s41598-021-81733-3.pdf"},"version_id":"4798743c-4c23-4447-95b9-bf2ed0af6dbd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Membrane lipids","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phospholipids","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth."}]},"item_type_id":"4","owner":"11","path":["79"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-02-10"},"publish_date":"2021-02-10","publish_status":"0","recid":"3796","relation_version_is_last":true,"title":["Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T11:39:06.283563+00:00"}