@article{oai:shiga-med.repo.nii.ac.jp:00003778,
 author = {岩佐, 磨佐紀 and 藤城, 綾 and 前川, 平 and 安藤, 朗 and IWASA, Masaki and FUJII, Sumie and FUJISHIRO, Aya and MAEKAWA, Taira and ANDOH, Akira and TAKAORI-KONDO, Akifumi and ICHINOHE, Tatsuo and MIURA, Yasuo},
 journal = {International Journal of Hematology},
 month = {Jan},
 note = {Two gray γ-irradiation is a widely employed basic module for total body irradiation (TBI) in allogeneic hematopoietic cell transplantation (HCT). The effects of γ-irradiation on hematopoietic and immune cells have been well investigated, but its effects on the bone marrow microenvironment (BMM) are unknown. Given the crucial contribution of mesenchymal/stromal stem cells (MSCs) in the BMM to hematopoiesis and osteogenesis, we investigated whether γ-irradiation affects the hallmark characteristics of human bone marrow-derived MSCs (BM-MSCs). Expansion of 2 Gy γ-irradiated BM-MSCs was delayed but eventually recovered. Colony formation and osteogenic, adipogenic, and chondrogenic differentiation capabilities of these cells were extensively suppressed. Irradiation of BM-MSCs did not affect the expansion of CD34 + hematopoietic stem and progenitor cells or production of CD11b + mature myeloid cells in co-cultures. However, it reduced production of CD19 + B-cells, as well as expression of CXCL12 and interleukin-7, which are essential for B-cell lymphopoiesis, in 2 Gy γ-irradiated BM-MSCs. Collectively, colony formation, osteogenic differentiation, and B-cell lymphopoiesis-supportive capabilities of γ-irradiated BM-MSCs were reduced. These effects may predispose survivors receiving HCT with TBI to defective bone formation and a perturbed humoral immune response., Journal Article},
 title = {Impact of 2 Gy γ-irradiation on the hallmark characteristics of human bone marrow-derived MSCs.},
 year = {2021}
}