{"created":"2023-06-19T10:43:29.577571+00:00","id":3721,"links":{},"metadata":{"_buckets":{"deposit":"595db6b0-661d-4d9e-ae40-c5e13248762e"},"_deposit":{"created_by":11,"id":"3721","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3721"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003721","sets":["19:206:207","19:232:233","30:68:267"]},"author_link":["7347","8243","7488","7491","2545","8241","1957","7826","8242","7600"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-10-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"18044","bibliographicVolumeNumber":"10","bibliographic_titles":[{},{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森宗, 孝夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"塚村, 篤史"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田埜, 郁実"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田中, 雄也"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"森, 康博"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山元, 武文"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西村, 正樹"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"遠山, 育夫"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"森, 雅樹"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cell competition is a cell-cell interaction mechanism which maintains tissue homeostasis through selective elimination of unfit cells. During early brain development, cells are eliminated through apoptosis. How cells are selected to undergo elimination remains unclear. Here we aimed to identify a role for cell competition in the elimination of suboptimal cells using an in vitro neuroepithelial model. Cell competition was observed when neural progenitor HypoE-N1 cells expressing RASV12 were surrounded by normal cells in the co-culture. The elimination through apoptosis was observed by cellular changes of RASV12 cells with rounding/fragmented morphology, by SYTOX blue-positivity, and by expression of apoptotic markers active caspase-3 and cleaved PARP. In this model, expression of juvenility-associated genes Srsf7 and Ezh2 were suppressed under cell-competitive conditions. Srsf7 depletion led to loss of cellular juvenescence characterized by suppression of Ezh2, cell growth impairment and enhancement of senescence-associated proteins. The cell bodies of eliminated cells were engulfed by the surrounding cells through phagocytosis. Our data indicates that neuroepithelial cell competition may have an important role for maintaining homeostasis in the neuroepithelium by eliminating suboptimal cells through loss of cellular juvenescence. ","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Research"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"33093561","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1038/s41598-020-74874-4"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1038/s41598-020-74874-4","subitem_relation_type_select":"DOI"}}]},"item_4_relation_13":{"attribute_name":"PMCID","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"PMC7582913"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7582913/","subitem_relation_type_select":"URI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s) 2020"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"20452322","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Jam, Faidruz Azura Binti"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morimune, Takao"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUKAMURA, Atsushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tano, Ayami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TANAKA, Yuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MORI, Yasuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMAMOTO, Takefumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"NISHIMURA, Masaki"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"TOOYAMA, Ikuo"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"MORI, Masaki"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-06"}],"displaytype":"detail","filename":"s41598-020-74874-4.pdf","filesize":[{"value":"3.5 MB"}],"format":"application/pdf","license_note":"This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"s41598-020-74874-4","url":"https://shiga-med.repo.nii.ac.jp/record/3721/files/s41598-020-74874-4.pdf"},"version_id":"c75c7c46-6a9d-4ed3-9c34-e68467132afb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cell biology","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell proliferation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Neuroepithelial cell competition triggers loss of cellular juvenescence.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Neuroepithelial cell competition triggers loss of cellular juvenescence."}]},"item_type_id":"4","owner":"11","path":["207","233","267"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-06"},"publish_date":"2020-11-06","publish_status":"0","recid":"3721","relation_version_is_last":true,"title":["Neuroepithelial cell competition triggers loss of cellular juvenescence."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T11:55:37.210217+00:00"}