{"created":"2023-06-19T10:43:19.070030+00:00","id":3538,"links":{},"metadata":{"_buckets":{"deposit":"d0f2b961-93ea-4baa-ac20-3319d12b9166"},"_deposit":{"created_by":11,"id":"3538","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3538"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003538","sets":["30:66:216","77:263:264"]},"author_link":["7887","7884","7885","564","7886","798","7888","7889"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-04-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"111","bibliographicVolumeNumber":"17","bibliographic_titles":[{},{"bibliographic_title":"Journal of neuroinflammation","bibliographic_titleLang":"en"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"辻, 俊一郎"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"村上, 節"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND:\nNeuroinflammation plays an important role in neonatal hypoxic-ischemic encephalopathy (HIE). Although microglia are largely responsible for injury-induced inflammatory response, they play beneficial roles in both normal and disease states. However, the effects of microglial depletion on neonatal HIE remain unclear.","subitem_description_type":"Abstract"},{"subitem_description":"METHODS:\nTamoxifen was administered to Cx3cr1CreER/+Rosa26DTA/+ (microglia-depleted model) and Cx3cr1CreER/+Rosa26DTA/- (control) mice at P8 and P9 to assess the effect of microglial depletion. The density of microglia was quantified using Iba-1 staining. Moreover, the proportion of resident microglia after the HI insult was analyzed using flow cytometric analysis. At P10, the HI insult was conducted using the Rice-Vannucci procedure at P10. The infarct size and apoptotic cells were analyzed at P13. Cytokine analyses were performed using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA) at P13.","subitem_description_type":"Abstract"},{"subitem_description":"RESULTS:\nAt P10, tamoxifen administration induced > 99% microglial depletion in DTA+ mice. Following HI insult, there was persisted microglial depletion over 97% at P13. Compared to male DTA- mice, male DTA+ mice exhibited significantly larger infarct volumes; however, there were no significant differences among females. Moreover, compared to male DTA- mice, male DTA+ mice had a significantly higher density of TUNEL+ cells in the caudoputamen, cerebral cortex, and thalamus. Moreover, compared to female DTA- mice, female DTA+ mice showed a significantly greater number of TUNEL+ cells in the hippocampus and thalamus. Compared to DTA- mice, ELISA revealed significantly lower IL-10 and TGF-β levels in both male and female DTA+ mice under both normal conditions and after HI (more pronounced).","subitem_description_type":"Abstract"},{"subitem_description":"CONCLUSION:\nWe established a microglial depletion model that aggravated neuronal damage and apoptosis after the HI insult, which was predominantly observed in males.","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"32276642","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1186/s12974-020-01792-7"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1186/s12974-020-01792-7","subitem_relation_type_select":"DOI"}}]},"item_4_relation_13":{"attribute_name":"PMCID","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"PMC7149909 "}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149909/","subitem_relation_type_select":"URI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© The Author(s). 2020"},{"subitem_rights":" This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data."}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1742-2094","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TSUJI, Shunichiro"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"DI MARTINO, Elena"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MUKAI, Takeo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"TSUJI, Shoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MURAKAMI, Takashi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"HARRIS, Robert A "}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"BLOMGREN, Klas"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"ÅDEN, Ulrika"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-01"}],"displaytype":"detail","filename":"s12974-020-01792-7.pdf","filesize":[{"value":"9.6 MB"}],"format":"application/pdf","license_note":"© The Author(s). 2020\n\n","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"s12974-020-01792-7","url":"https://shiga-med.repo.nii.ac.jp/record/3538/files/s12974-020-01792-7.pdf"},"version_id":"1e55104b-484f-4592-9f10-101184d93d8f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hypoxic-ischemic encephalopathy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Ischemic stroke","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Microglia","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Neonate","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Aggravated brain injury after neonatal hypoxic ischemia in microglia-depleted mice.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Aggravated brain injury after neonatal hypoxic ischemia in microglia-depleted mice."}]},"item_type_id":"4","owner":"11","path":["216","264"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-05-01"},"publish_date":"2020-05-01","publish_status":"0","recid":"3538","relation_version_is_last":true,"title":["Aggravated brain injury after neonatal hypoxic ischemia in microglia-depleted mice."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T12:20:00.032336+00:00"}