{"created":"2023-06-19T10:43:03.579874+00:00","id":3176,"links":{},"metadata":{"_buckets":{"deposit":"3e77695d-c8b8-49d5-813d-13e6de37398e"},"_deposit":{"created_by":11,"id":"3176","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3176"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00003176","sets":["77:78:79"]},"author_link":["7156","4010","675"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-22","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"306","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"Scientific Reports"}]}]},"item_4_creator_3":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"池田, 義人"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"森田, 真也"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"寺田, 智祐"}],"nameIdentifiers":[{},{}]}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bile salts have potent detergent properties and damaging effects on cell membranes, leading to liver injury. However, the molecular mechanisms for the protection of hepatocytes against bile salts are not fully understood. In this study, we demonstrated that the cytotoxicity of nine human major bile salts to HepG2 cells and primary human hepatocytes was prevented by phosphatidylcholine (PC). In contrast, cholesterol had no direct cytotoxic effects but suppressed the cytoprotective effects of PC. PC reduced the cell-association of bile salt, which was reversed by cholesterol. Light scattering measurements and gel filtration chromatography revealed that cholesterol within bile salt/PC dispersions decreased mixed micelles but increased vesicles, bile salt simple micelles and monomers. These results suggest that cholesterol attenuates the cytoprotective effects of PC against bile salts by facilitating the formation of bile salt simple micelles and monomers. Therefore, biliary PC and cholesterol may play different roles in the pathogenesis of bile salt-induced liver injury.","subitem_description_type":"Abstract"}]},"item_4_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature"}]},"item_4_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"28331225","subitem_relation_type_select":"PMID"}}]},"item_4_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/s41598-017-00476-2","subitem_relation_type_select":"DOI"}}]},"item_4_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2045-2322","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"IKEDA, Yoshito"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"森田, 真也"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"TERADA, Tomohiro"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-07"}],"displaytype":"detail","filename":"s41598-017-00476-2.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","license_note":"This work is licensed under a Creative Commons Attribution 4.0 International \nLicense. The images or other third party material in this article are included in\nthe article’s Creative Commons license, unless indicated otherwise in the credit\nline; if the material is not included under the Creative Commons license,\nusers will need to obtain permission from the license holder to reproduce the\nmaterial. To view a copy of this license, visit http://creativecommons.org\n/licenses/by/4.0/","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"s41598-017-00476-2","url":"https://shiga-med.repo.nii.ac.jp/record/3176/files/s41598-017-00476-2.pdf"},"version_id":"e2d384e7-695d-4b36-966f-3988c792d949"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cholesterol attenuates cytoprotective effects of phosphatidylcholine against bile salts.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cholesterol attenuates cytoprotective effects of phosphatidylcholine against bile salts."}]},"item_type_id":"4","owner":"11","path":["79"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-09-07"},"publish_date":"2017-09-07","publish_status":"0","recid":"3176","relation_version_is_last":true,"title":["Cholesterol attenuates cytoprotective effects of phosphatidylcholine against bile salts."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-06-19T12:16:06.343087+00:00"}