{"created":"2023-06-19T10:42:51.553603+00:00","id":2897,"links":{},"metadata":{"_buckets":{"deposit":"adbf5ef8-7523-4138-ad32-3aa382562765"},"_deposit":{"created_by":11,"id":"2897","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2897"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00002897","sets":["10:37"]},"author_link":[],"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"343","bibliographicPageStart":"337","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"Experimental Animals","bibliographic_titleLang":"en"}]}]},"item_6_date_granted_70":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2017-09-13"}]},"item_6_degree_grantor_68":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"滋賀医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14202","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_67":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Transient receptor potential cation channel subfamily M member 8 (TRPM8) is associated with sensitivity to cold sensation in mammals. A previous study demonstrated that TRPM8 was overexpressed in the skin of ovariectomized (OVX) rats due to the loss of estrogen. In the present study, we investigated whether estrogen replacement restricts overexpression of the TRPM8 channel in the skin of OVX rats. We divided 15 Sprague Dawley rats into three groups: a non-operated group (NON-OPE), an ovariectomy group (OVX), and a group subjected to estrogen replacement during 4 weeks beginning 7 days after ovariectomy (OVX + E2). Five weeks later, TRPM8 channel mRNA and protein in lumbar skin were quantified by real-time RT-PCR, protein ELISA, and immunohistochemistry. The OVX + E2 group exhibited a trend for decreased expression of the TRPM8 channel in the lumbar skin in comparison with the OVX group, whereas ELISA data and immunohistochemistry data and immunohistochemistry graphs relating to TRPM8 protein did not show any obvious differences between the OVX group and the OVX + E2 group. Estrogen replacement may restrict the overexpression of TRPM8 in the dermis of OVX rats. ","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Thesis or Dissertation","subitem_description_type":"Other"}]},"item_6_description_69":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"平成29年度","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_71":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"乙第433号"}]},"item_6_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Association for Laboratory Animal Science","subitem_publisher_language":"en"}]},"item_6_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"28626113","subitem_relation_type_select":"PMID"}}]},"item_6_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1538/expanim.17-0028"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1538/expanim.17-0028","subitem_relation_type_select":"DOI"}}]},"item_6_relation_13":{"attribute_name":"本文","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"J-STAGE"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/expanim/66/4/66_17-0028/_article","subitem_relation_type_select":"URI"}}]},"item_6_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1341-1357","subitem_source_identifier_type":"PISSN"}]},"item_6_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"KUBO, Takuro","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-17"}],"displaytype":"detail","filename":"66_17-0028.pdf","filesize":[{"value":"822.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"s12920-015-0080-6.pdf","objectType":"fulltext","url":"https://shiga-med.repo.nii.ac.jp/record/2897/files/66_17-0028.pdf"},"version_id":"72f2e12e-490d-441c-82eb-3bcbe9a15611"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cold-sensitive TRPM8 channel receptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"estrogen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"estrogen replacement","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"oversensitivity to cold","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"post-menopause","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Effects of β-estradiol on cold-sensitive receptor channel TRPM8 in ovariectomized rats.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Effects of β-estradiol on cold-sensitive receptor channel TRPM8 in ovariectomized rats.","subitem_title_language":"en"}]},"item_type_id":"6","owner":"11","path":["37"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-04-23"},"publish_date":"2018-04-23","publish_status":"0","recid":"2897","relation_version_is_last":true,"title":["Effects of β-estradiol on cold-sensitive receptor channel TRPM8 in ovariectomized rats."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-09-15T05:16:57.809956+00:00"}