{"created":"2023-06-19T10:42:51.282492+00:00","id":2891,"links":{},"metadata":{"_buckets":{"deposit":"b22a0597-cb01-4fdc-9711-827320266cf2"},"_deposit":{"created_by":11,"id":"2891","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2891"},"status":"published"},"_oai":{"id":"oai:shiga-med.repo.nii.ac.jp:00002891","sets":["10:37"]},"author_link":[],"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-07-16","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageStart":"53","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Cancer cell international","bibliographic_titleLang":"en"}]}]},"item_6_date_granted_70":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-10"}]},"item_6_degree_grantor_68":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"滋賀医科大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14202","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_6_degree_name_67":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）","subitem_degreename_language":"ja"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background:\nEffective therapies for early endometrial cancer usually involve surgical excision and consequent infertility Therefore, new treatment approaches that preserve fertility should be developed. Metformin, a well-tolerated anti-diabetic drug, can inhibit cancer cell growth. However, the mechanism of metformin action is not well understood. Here we investigate the roles of autophagy and apoptosis in the anti-cancer effects of metformin on endometrial cancer cells. ","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"Methods:\nIshikawa endometrial cancer cells were treated with metformin. WST-8 assays, colony formation assays, flow cytometry, caspase luminescence measurement, immunofluorescence, and western blots were used to assess the effects of metformin on cell viability, proliferation, cell cycle progression, apoptosis, and autophagy. ","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"Results:\nMetformin-treated cells exhibited significantly lower viability and proliferation and significantly more cell cycle arrest in G1 and G2/M than control cells. These cells also exhibited significantly more apoptosis via both intrinsic and extrinsic pathways. In addition, metformin treatment induced autophagy. Inhibition of autophagy, either by Beclin1 knockdown or by 3-methyladenine-mediated inhibition of caspase-3/7, suppressed the anti-proliferative effects of metformin on endometrial cancer cells. These findings indicate that the anti-proliferative effects and apoptosis caused by metformin are partially or completely dependent on autophagy. ","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"Conclusions:\nWe showed that metformin suppresses endometrial cancer cell growth via cell cycle arrest and concomitant autophagy and apoptosis. ","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_6_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Thesis or Dissertation","subitem_description_type":"Other"}]},"item_6_description_69":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"平成26年度","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_6_dissertation_number_71":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第730号"}]},"item_6_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"BioMed Central","subitem_publisher_language":"en"}]},"item_6_relation_10":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"24966801","subitem_relation_type_select":"PMID"}}]},"item_6_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"10.1186/1475-2867-14-53"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1186/1475-2867-14-53","subitem_relation_type_select":"DOI"}}]},"item_6_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1475-2867","subitem_source_identifier_type":"PISSN"}]},"item_6_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12050420","subitem_source_identifier_type":"NCID"}]},"item_6_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TAKAHASHI, Akimasa","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-17"}],"displaytype":"detail","filename":"1475-2867-14-53.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1475-2867-14-53.pdf","objectType":"fulltext","url":"https://shiga-med.repo.nii.ac.jp/record/2891/files/1475-2867-14-53.pdf"},"version_id":"ca590975-7e0a-4107-b68d-b46e6e43df85"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Metformin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Endometrial cancer","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Autophagy","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Metformin impairs growth of endometrial cancer cells via cell cycle arrest and concomitant autophagy and apoptosis.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Metformin impairs growth of endometrial cancer cells via cell cycle arrest and concomitant autophagy and apoptosis.","subitem_title_language":"en"}]},"item_type_id":"6","owner":"11","path":["37"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-07-23"},"publish_date":"2015-07-23","publish_status":"0","recid":"2891","relation_version_is_last":true,"title":["Metformin impairs growth of endometrial cancer cells via cell cycle arrest and concomitant autophagy and apoptosis."],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-09-15T05:16:54.265259+00:00"}