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Similarities and differences in metabolites of tongue cancer cells among two- and three-dimensional cultures and xenografts.
http://hdl.handle.net/10422/00012886
http://hdl.handle.net/10422/00012886949e305d-79cd-4602-ab7a-1ffe5321ca40
名前 / ファイル | ライセンス | アクション |
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cas.14749 (3.5 MB)
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This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-01-06 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Similarities and differences in metabolites of tongue cancer cells among two- and three-dimensional cultures and xenografts. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cancer metabolism | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | mitochondrial function | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | three-dimensional | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | tissueoid cell culture system | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | tongue cancer | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
MURAKAMI, Shoko
× MURAKAMI, Shoko× TANAKA, Hiroyuki× NAKAYAMA, Takahisa× TANIURA, Naoko× MIYAKE, Toru× TANI, Masaji× KUSHIMA, Ryoji× YAMAMOTO, Gaku× SUGIHARA, Hiroyuki× MUKAISHO, Ken-ichi |
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著者別名 |
村上, 翔子
× 村上, 翔子× 田中, 裕之× 仲山, 貴永× 三宅, 亨× 谷, 眞至× 九嶋, 亮治× 山本, 学× 杉原, 洋行× 向所, 賢一 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Metabolic programming of cancer cells is an essential step in transformation and tumor growth. We established two-dimensional (2D) monolayer and three-dimensional (3D) cultures, the latter called a "tissueoid cell culture system", using four types of tongue cancer cell lines. We also undertook a comprehensive metabolome analysis of three groups that included xenografts created by transplanting the cell lines into nude mice. In addition, we undertook a functional analysis of the mitochondria, which plays a key role in cancer metabolism. Principal component analysis revealed the plots of the four cell lines to be much narrower in 2D culture than in 3D culture and xenograft groups. Moreover, compared to xenografts, the 2D culture had significantly lower levels of most metabolites. These results suggest that the unique characteristics of each cell disappeared in 2D culture, and a type of metabolism unique to monolayer culture took over. Conversely, ATP production, biomass synthesis, and maintenance of redox balance were shown in 3D culture using sufficient nutrients, which closely resembled the metabolic activity in the xenografts. However, there were several differences between the metabolic activity in the 3D culture and xenografts. In vivo, the cancer tissue had blood flow with stromal cells present around the cancer cells. In the xenografts, we detected metabolized and degraded products in the liver and other organs of the host mice. Furthermore, the 3D system did not show impairment of mitochondrial function in the cancer cells, suggesting that cancer cells produce energy simultaneously through mitochondria, as well as aerobic glycolysis. | |||||
言語 | en | |||||
書誌情報 |
en : Cancer science 巻 112, 号 2, p. 918-931, 発行日 2020-11-26 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Wiley | |||||
ISSN | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 1349-7006 | |||||
PMID | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 33244783 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1111/cas.14749 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | © 2020 The Authors. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |