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Optimization of 3D Immunofluorescence Analysis and Visualization Using IMARIS and MeshLab.
http://hdl.handle.net/10422/00013508
http://hdl.handle.net/10422/0001350894beb3d2-c4e3-4df3-8709-da0a8af913f9
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
tjem.2022.J106 (2.6 MB)
|
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license
(https://creativecommons.org/licenses/by/4.0/). |
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2023-03-08 | |||||
| タイトル | ||||||
| タイトル | Optimization of 3D Immunofluorescence Analysis and Visualization Using IMARIS and MeshLab. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | IMARIS | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | MeshLab | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | colocalization analysis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | 3D | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | decimation | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | simplification | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
BAKAR, Zulzikry Hafiz Abu
× BAKAR, Zulzikry Hafiz Abu× BELLIER, Jean-Pierre× WAN HGAH, Wan Zurinah× YANAGISAWA, Daijiro× MUKAISHO, Ken-ichi× TOOYAMA, Ikuo |
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| 著者別名 |
柳沢, 大治郎
× 柳沢, 大治郎× 向所, 賢一× 遠山, 育夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The precision of colocalization analysis is enhanced by 3D and is potentially more accurate than 2D. Even though 3D improves the visualization of colocalization analysis, rendering a colocalization model may generate a model with numerous polygons. We developed a 3D colocalization model of FtMt/LC3 followed by simplification. Double immunofluorescence staining of FtMt and LC3 was conducted, and stacked images were acquired. We used IMARIS to render the 3D colocalization model of FtMt/LC3 and further processed it with MeshLab to decimate and generate a less complex colocalization model. We examined the available simplification algorithm using MeshLab in detail and evaluated the feasibility of each procedure in generating a model with less complexity. The quality of the simplified model was subsequently assessed. MeshLab's available shaders were scrutinized to facilitate the spatial colocalization determination. Finally, we showed that QECD was the most effective method for reducing the polygonal complexity of the colocalization model without compromising its quality. In addition, we would recommend implementing the x-ray shader, which we found useful for visualizing colocalization. As 3D was found to be more accurate in quantifying colocalization, our study provides a novel and dependable method for rendering 3D models for colocalization analysis. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Cells 巻 12, 号 2, 発行日 2023-01-04 |
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| 出版者 | ||||||
| 出版者 | MDPI | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 2073-4409 | |||||
| PMID | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 36672153 | |||||
| PMCID | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/pmc/articles/pmc9856541/ | |||||
| 言語 | en | |||||
| 関連名称 | PMC9856541 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.3390/cells12020218 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
