WEKO3
アイテム
{"_buckets": {"deposit": "2d9d708a-855f-4ad1-b407-8c4233f3ec74"}, "_deposit": {"created_by": 11, "id": "4082", "owners": [11], "pid": {"revision_id": 0, "type": "depid", "value": "4082"}, "status": "published"}, "_oai": {"id": "oai:shiga-med.repo.nii.ac.jp:00004082", "sets": ["192", "268", "274"]}, "author_link": ["3912", "1961", "7872", "7839", "8800", "1857", "7833", "8801", "1971", "8803", "7832", "7840", "8802"], "item_4_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2022-03-17", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "86", "bibliographicPageStart": "77", "bibliographicVolumeNumber": "24", "bibliographic_titles": [{}, {"bibliographic_title": "Molecular Therapy - Oncolytics", "bibliographic_titleLang": "en"}]}]}, "item_4_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "寺田, 晃士"}], "nameIdentifiers": [{"nameIdentifier": "7832", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70342722", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70342722"}]}, {"creatorNames": [{"creatorName": "近藤, 健太"}], "nameIdentifiers": [{"nameIdentifier": "7833", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60779974", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60779974"}]}, {"creatorNames": [{"creatorName": "石垣, 宏仁"}], "nameIdentifiers": [{"nameIdentifier": "1857", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90432301", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90432301"}]}, {"creatorNames": [{"creatorName": "里岡, 大樹"}], "nameIdentifiers": [{"nameIdentifier": "7872", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "40750360", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=40750360"}]}, {"creatorNames": [{"creatorName": "平田, 多佳子"}], "nameIdentifiers": [{"nameIdentifier": "3912", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "00346199", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=00346199"}, {"nameIdentifier": "0000-0002-1740-6168", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "https://orcid.org/0000-0002-1740-6168"}]}, {"creatorNames": [{"creatorName": "小笠原, 一誠"}], "nameIdentifiers": [{"nameIdentifier": "1971", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20169163", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=20169163"}]}, {"creatorNames": [{"creatorName": "伊藤, 靖"}], "nameIdentifiers": [{"nameIdentifier": "1961", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90324566", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90324566"}]}, {"creatorNames": [{"creatorName": "縣, 保年"}], "nameIdentifiers": [{"nameIdentifier": "7840", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60263141", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60263141"}]}]}, "item_4_description_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "pdf", "subitem_description_type": "Other"}]}, "item_4_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "To develop effective adoptive cell transfer therapy using T cell receptor (TCR)-engineered T cells, it is critical to isolate tumor-reactive TCRs that have potent anti-tumor activity. In humans, tumor-infiltrating lymphocytes (TILs) have been reported to contain CD8+PD-1+ T cells that express tumor-reactive TCRs. Characterization of tumor reactivity of TILs from non-human primate tumors could improve anti-tumor activity of TCR-engineered T cells in preclinical research. In this study, we sought to isolate TCR genes from CD8+PD-1+ T cells among TILs in a cynomolgus macaque model of tumor transplantation in which the tumors were infiltrated with CD8+ T cells and were eventually rejected. We analyzed the repertoire of TCRα and β pairs obtained from single CD8+PD-1+ T cells in TILs and circulating lymphocytes and identified multiple TCR pairs with high frequency, suggesting that T cells expressing these recurrent TCRs were clonally expanded in response to tumor cells. We further showed that the recurrent TCRs exhibited cytotoxic activity to tumor cells in vitro and potent anti-tumor activity in mice transplanted with tumor cells. These results imply that this tumor transplantation macaque model recapitulates key features of human TILs and can serve as a platform toward preclinical studies of non-human primate tumor models.", "subitem_description_type": "Abstract"}]}, "item_4_description_42": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_description": "Journal Article", "subitem_description_type": "Other"}]}, "item_4_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Cell Press"}]}, "item_4_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.omto.2021.12.003"}], "subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.omto.2021.12.003", "subitem_relation_type_select": "DOI"}}]}, "item_4_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "©2021 The Authors."}]}, "item_4_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2372-7705", "subitem_source_identifier_type": "ISSN"}]}, "item_4_version_type_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "TERADA, Koji"}], "nameIdentifiers": [{"nameIdentifier": "7832", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70342722", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70342722"}]}, {"creatorNames": [{"creatorName": "KONDO, Kenta"}], "nameIdentifiers": [{"nameIdentifier": "7833", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60779974", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60779974"}]}, {"creatorNames": [{"creatorName": "ISHIGAKI, Hirohito"}], "nameIdentifiers": [{"nameIdentifier": "1857", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90432301", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90432301"}]}, {"creatorNames": [{"creatorName": "NAGASHIMA, Ayaka"}], "nameIdentifiers": [{"nameIdentifier": "8800", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SATOOKA, Hiroki"}], "nameIdentifiers": [{"nameIdentifier": "7872", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "40750360", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=40750360"}]}, {"creatorNames": [{"creatorName": "NAGANO, Seiji"}], "nameIdentifiers": [{"nameIdentifier": "8801", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MASUDA, Kyoko"}], "nameIdentifiers": [{"nameIdentifier": "8802", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KAWAMURA, Teruhisa"}], "nameIdentifiers": [{"nameIdentifier": "8803", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HIRATA, Takako"}], "nameIdentifiers": [{"nameIdentifier": "3912", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "00346199", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=00346199"}, {"nameIdentifier": "0000-0002-1740-6168", "nameIdentifierScheme": "ORCID", "nameIdentifierURI": "https://orcid.org/0000-0002-1740-6168"}]}, {"creatorNames": [{"creatorName": "OGASAWARA, Kazumasa"}], "nameIdentifiers": [{"nameIdentifier": "1971", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20169163", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=20169163"}]}, {"creatorNames": [{"creatorName": "ITOH, Yasushi"}], "nameIdentifiers": [{"nameIdentifier": "1961", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90324566", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90324566"}]}, {"creatorNames": [{"creatorName": "KAWAMOTO, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "7839", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "AGATA, Yasutoshi"}], "nameIdentifiers": [{"nameIdentifier": "7840", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60263141", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60263141"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-01-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "j.omto.2021.12.003.pdf", "filesize": [{"value": "2.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "This is an open access article under the CC BY-NC-ND license \n(http://creativecommons.org/licenses/by-nc-nd/4.0/).", "licensetype": "license_free", "mimetype": "application/pdf", "size": 2100000.0, "url": {"label": "j.omto.2021.12.003", "url": "https://shiga-med.repo.nii.ac.jp/record/4082/files/j.omto.2021.12.003.pdf"}, "version_id": "16674d75-fa47-46f6-82e4-ee02b01c7e47"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "adoptive cell transfer", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "T cell receptor-engineered T cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "tumor-infiltrating lymphocytes", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "non-human primates", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "iPS cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "regenerated T cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cytotoxic T lymphocytes (CTLs)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "macaques", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PD-1", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "TCR repertoire", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Isolation of TCR genes with tumor-killing activity from tumor-infiltrating and circulating lymphocytes in a tumor rejection cynomolgus macaque model", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Isolation of TCR genes with tumor-killing activity from tumor-infiltrating and circulating lymphocytes in a tumor rejection cynomolgus macaque model"}]}, "item_type_id": "4", "owner": "11", "path": ["192", "268", "274"], "permalink_uri": "http://hdl.handle.net/10422/00013178", "pubdate": {"attribute_name": "公開日", "attribute_value": "2022-01-04"}, "publish_date": "2022-01-04", "publish_status": "0", "recid": "4082", "relation": {}, "relation_version_is_last": true, "title": ["Isolation of TCR genes with tumor-killing activity from tumor-infiltrating and circulating lymphocytes in a tumor rejection cynomolgus macaque model"], "weko_shared_id": -1}
Isolation of TCR genes with tumor-killing activity from tumor-infiltrating and circulating lymphocytes in a tumor rejection cynomolgus macaque model
http://hdl.handle.net/10422/00013178
http://hdl.handle.net/10422/000131783d1c9562-dcdf-48e3-b35b-6ee7372e524f
名前 / ファイル | ライセンス | アクション |
---|---|---|
j.omto.2021.12.003 (2.1 MB)
|
This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/by-nc-nd/4.0/). |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-01-04 | |||||
タイトル | ||||||
タイトル | Isolation of TCR genes with tumor-killing activity from tumor-infiltrating and circulating lymphocytes in a tumor rejection cynomolgus macaque model | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | adoptive cell transfer | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | T cell receptor-engineered T cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | tumor-infiltrating lymphocytes | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | non-human primates | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | iPS cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | regenerated T cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cytotoxic T lymphocytes (CTLs) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | macaques | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | PD-1 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | TCR repertoire | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
TERADA, Koji
× TERADA, Koji× KONDO, Kenta× ISHIGAKI, Hirohito× NAGASHIMA, Ayaka× SATOOKA, Hiroki× NAGANO, Seiji× MASUDA, Kyoko× KAWAMURA, Teruhisa× HIRATA, Takako× OGASAWARA, Kazumasa× ITOH, Yasushi× KAWAMOTO, Hiroshi× AGATA, Yasutoshi |
|||||
著者別名 |
寺田, 晃士
× 寺田, 晃士× 近藤, 健太× 石垣, 宏仁× 里岡, 大樹× 平田, 多佳子× 小笠原, 一誠× 伊藤, 靖× 縣, 保年 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To develop effective adoptive cell transfer therapy using T cell receptor (TCR)-engineered T cells, it is critical to isolate tumor-reactive TCRs that have potent anti-tumor activity. In humans, tumor-infiltrating lymphocytes (TILs) have been reported to contain CD8+PD-1+ T cells that express tumor-reactive TCRs. Characterization of tumor reactivity of TILs from non-human primate tumors could improve anti-tumor activity of TCR-engineered T cells in preclinical research. In this study, we sought to isolate TCR genes from CD8+PD-1+ T cells among TILs in a cynomolgus macaque model of tumor transplantation in which the tumors were infiltrated with CD8+ T cells and were eventually rejected. We analyzed the repertoire of TCRα and β pairs obtained from single CD8+PD-1+ T cells in TILs and circulating lymphocytes and identified multiple TCR pairs with high frequency, suggesting that T cells expressing these recurrent TCRs were clonally expanded in response to tumor cells. We further showed that the recurrent TCRs exhibited cytotoxic activity to tumor cells in vitro and potent anti-tumor activity in mice transplanted with tumor cells. These results imply that this tumor transplantation macaque model recapitulates key features of human TILs and can serve as a platform toward preclinical studies of non-human primate tumor models. | |||||
書誌情報 |
en : Molecular Therapy - Oncolytics 巻 24, p. 77-86, 発行日 2022-03-17 |
|||||
出版者 | ||||||
出版者 | Cell Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2372-7705 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.omto.2021.12.003 | |||||
関連名称 | 10.1016/j.omto.2021.12.003 | |||||
権利 | ||||||
権利情報 | ©2021 The Authors. | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | ||||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal Article |