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Receptor-Mediated Bioassay Reflects Dynamic Change of Glucose-Dependent Insulinotropic Polypeptide by Dipeptidyl Peptidase 4 Inhibitor Treatment in Subjects With Type 2 Diabetes.
http://hdl.handle.net/10422/00012720
http://hdl.handle.net/10422/000127205133a59c-7f46-4ad5-a2be-37bef56cecae
名前 / ファイル | ライセンス | アクション |
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fendo.2020.00214 (728.1 kB)
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© 2020 Yanagimachi, Fujita, Takeda, Honjo, Yokoyama and Haneda.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-06-17 | |||||
タイトル | ||||||
タイトル | Receptor-Mediated Bioassay Reflects Dynamic Change of Glucose-Dependent Insulinotropic Polypeptide by Dipeptidyl Peptidase 4 Inhibitor Treatment in Subjects With Type 2 Diabetes. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | dipeptidyl peptidase 4 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | glucagon | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | glucagon-like peptide 1 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | glucose-dependent insulinotropic polypeptide | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | receptor-mediated bioassay | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
YANAGIMACHI, Tsuyoshi
× YANAGIMACHI, Tsuyoshi× FUJITA, Yukihiro× TAKEDA, Yasutaka× HONJO, Jun× YOKOYAMA, Hiroki× HANEDA, Masakazu |
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著者別名 |
藤田, 征弘
× 藤田, 征弘 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Objective: We recently observed a greater increase in plasma levels of bioactive glucose-dependent insulinotropic polypeptide (GIP) than glucagon-like peptide 1 (GLP-1) using the receptor-mediated bioassays in the subjects with normal glycemic tolerance (NGT) treated with dipeptidyl peptidase 4 (DPP-4) inhibitors, which may be unappreciated using conventional enzyme-linked immunosorbent assays (ELISAs) during oral glucose tolerance test. Thus, we determined incretin levels in addition to glucagon level using the bioassays in type 2 diabetes mellitus (T2DM) subjects with or without treatment of DPP-4 inhibitor, to evaluate whether these assays can accurately measure bioactivity of these peptides. |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Methods: We performed single meal tolerance test (MTT) by using a cookie meal (carbohydrate 75.0 g, protein 8.0 g, fat 28.5 g) in the subjects with NGT (n = 9), the subjects with T2DM treated without DPP-4 inhibitor (n = 7) and the subjects with T2DM treated with DPP-4 inhibitor (n = 10). All subjects fasted for 10-12 h before the MTT, and blood samples were collected at 0, 30, 60, and 120 min. We used the cell lines stably cotransfected with human-form GIP, GLP-1 or glucagon receptor, and a cyclic adenosine monophosphate-inducible luciferase expression construct for the bioassays. We measured active GIP, active GLP-1, and glucagon by the bioassays. To evaluate the efficacy of bioassay, we measured identical samples via ELISA kits. |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Results: During the single MTT study, postprandial active GIP bioassay levels of T2DM with DPP-4 inhibitor treatment were drastically higher than those of NGT and T2DM without DPP-4 inhibitor, although the DPP-4 inhibitor-treated group showed moderate increase of active GIPELISA and active GLP-1 bioassay , while active GLP-1 bioassay levels of T2DM subjects without DPP-4 inhibitor were comparable to those of NGT subjects. During the serial MTT, administration of DPP-4 inhibitor significantly increased active GIP bioassay levels, but not active GLP-1 bioassay . |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Conclusions: In comparison to conventional ELISA, receptor-mediated bioassay reflects dynamic change of GIP polypeptide by DPP-4 inhibitor treatment in subjects with type 2 diabetes. |
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書誌情報 |
en : Frontiers in Endocrinology 巻 11, 発行日 2020-04-24 |
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出版者 | ||||||
出版者 | Frontiers Research Foundation | |||||
PMCID | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7193081/ | |||||
関連名称 | PMC7193081 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3389/fendo.2020.00214 | |||||
関連名称 | 10.3389/fendo.2020.00214 | |||||
権利 | ||||||
権利情報 | © 2020 Yanagimachi, Fujita, Takeda, Honjo, Yokoyama and Haneda. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal Article |