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Extracellular and intracellular mechanisms of mechanotransduction in three-dimensionally embedded rat chondrocytes.
http://hdl.handle.net/10422/00012410
http://hdl.handle.net/10422/0001241023417112-cae0-41b2-8e80-aed1fb0d7045
名前 / ファイル | ライセンス | アクション |
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journal.pone.0114327 (987.7 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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公開日 | 2018-04-23 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | Extracellular and intracellular mechanisms of mechanotransduction in three-dimensionally embedded rat chondrocytes. | |||||||
言語 | ||||||||
言語 | eng | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
資源タイプ | doctoral thesis | |||||||
アクセス権 | ||||||||
アクセス権 | open access | |||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
著者 |
SHIOJI, Suguru
× SHIOJI, Suguru
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抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | Purpose: Articular cartilage homeostasis involves modulation of chondrocyte matrix synthesis in response to mechanical stress (MS). We studied extracellular and intracellular mechanotransduction pathways mediating this response. |
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言語 | en | |||||||
抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | Methods: We first confirmed rapid up-regulation of the putative chondro-protective cytokine, interleukin (IL)-4, as an immediate response to MS. We then studied the role of IL-4 by investigating responses to exogenous IL-4 or a specific IL-4 inhibitor, combined with MS. Next we investigated the intracellular second messengers. Since chondrocyte phenotype alters according to the extracellular environment, we characterized the response to mechanotransduction in 3-dimensionally embedded chondrocytes. |
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言語 | en | |||||||
抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | Results: Expression of aggrecan and type II collagen was significantly up-regulated by exogenous IL-4 whereas MS-induced matrix synthesis was inhibited by an IL-4 blocker. Further, MS-induced matrix synthesis was completely blocked by a p38 MAPK inhibitor, while it was only partially blocked by inhibitors of other putative second messengers. |
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言語 | en | |||||||
抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | Conclusion: IL-4 mediates an extracellular pathway of mechanotransduction, perhaps via an autocrine/paracrine loop, while p38 mediates an intracellular pathway prevalent only in a 3-dimensional environment. |
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言語 | en | |||||||
学位名 | ||||||||
言語 | ja | |||||||
学位名 | 博士(医学) | |||||||
学位授与機関 | ||||||||
学位授与機関識別子Scheme | kakenhi | |||||||
学位授与機関識別子 | 14202 | |||||||
言語 | ja | |||||||
学位授与機関名 | 滋賀医科大学 | |||||||
学位授与年度 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | 平成29年度 | |||||||
言語 | ja | |||||||
学位授与年月日 | ||||||||
学位授与年月日 | 2017-09-13 | |||||||
学位授与番号 | ||||||||
学位授与番号 | 乙第434号 | |||||||
書誌情報 |
en : PLoS ONE 巻 9, 号 12, p. e114327, 発行日 2014-12-05 |
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出版者 | ||||||||
言語 | en | |||||||
出版者 | Public Library of Science | |||||||
著者版フラグ | ||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
ISSN | ||||||||
収録物識別子タイプ | EISSN | |||||||
収録物識別子 | 1932-6203 | |||||||
PMID | ||||||||
識別子タイプ | PMID | |||||||
関連識別子 | 25479057 | |||||||
DOI | ||||||||
識別子タイプ | DOI | |||||||
関連識別子 | https://doi.org/10.1371/journal.pone.0114327 | |||||||
関連名称 | 10.1371/journal.pone.0114327 | |||||||
資源タイプ | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | Thesis or Dissertation |