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Overexpression of protein kinase C β isoform by adenoviral gene transfer accelerated intimal hyperplasia in rat carotid artery
http://hdl.handle.net/10422/112
http://hdl.handle.net/10422/1120a96dd23-d7f0-4d1f-9f90-6e139c537213
名前 / ファイル | ライセンス | アクション |
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jsums16p041.pdf (977.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-04-12 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Overexpression of protein kinase C β isoform by adenoviral gene transfer accelerated intimal hyperplasia in rat carotid artery | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
その他のタイトル | ||||||
その他のタイトル | Protein kinase C βの過剰発現は,バルーン傷害後のラット頸動脈の内膜肥厚を促進する | |||||
言語 | ja | |||||
その他のタイトル | ||||||
その他のタイトル | Protein kinase C β ノ カジョウ ハツゲン ハ バルーン ショウガイゴ ノ ラット ケイドウミャク ノ ナイマク ヒコウ ヲ ソクシンスル | |||||
言語 | ja-Kana | |||||
著者 |
TAKAHARA, Hidenori
× TAKAHARA, Hidenori× TAKAHARA, Noriko× KING, George L.× FUJIMURA, Masaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Vigorous restenosis is responsible for high failure rates of angioplasty in diabetic patients. Sinceprotein kinase C (PKC)β isoform activation is associated with vascular complications of diabetes, we havedetermined whether PKCβ isoform overexpression can stimulate growth and migration of vascular smoothmuscle cells (SMCs) and cause an acceleration of arterial intimal hyperplasia. Overexpression of PKCβ1(adv-PKCβ1) or β-galactosidase (adv-βgal) were achieved using replication-deficient adenovirus vectors incultured rat aortic SMCs and rat carotid arteries after balloon injury. Rat aortic SMCs infected with adv-PKCβ1 at 109 PFU/ml increased PKC activity and PKCβ1 isoform protein level by more than 10-fold comparedto adv-βgal infected SMCs at 4 days after infection. The growth and migration rates in PKCβ1 overexpressedSMCs were increased by 1.5- to 1.9-fold compared to adv-βgal infected SMCs. Protein levels ofPKCβ1 isoform in adv-PKCβ1 infected arteries were increased by 4.2- and 2.0-fold compared to non-infectedand adv-βgal infected arteries, respectively, at 7 days after adenovirus infection. Ratio of intima/media area,a marker of restenosis, were 0.26±0.08 and 0.54±0.14 (p<0.05) at 7 days after infection in adv-βgal and adv-PKCβ1 infected arteries, respectively. Thus, overexpression of PKCβ isoform can enhance vascular SMCsmigration and growth, causing an accelerated rate of intimal hyperplasia, and may involve the restenoticprocess after angioplasty in diabetic patients. | |||||
言語 | en | |||||
書誌情報 |
ja : 滋賀医科大学雑誌 巻 16, p. 41-53, 発行日 2001-02 |
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出版者 | ||||||
言語 | ja | |||||
出版者 | 滋賀医科大学雑誌刊行会 | |||||
NCID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10044327 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |