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Application of "Tissueoid Cell Culture System" Using a Silicate Fiber Scaffold for Cancer Research.
http://hdl.handle.net/10422/00012813
http://hdl.handle.net/10422/000128139b467a2c-0ad2-4424-841a-c7f4d9cd6f62
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-10-13 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Application of "Tissueoid Cell Culture System" Using a Silicate Fiber Scaffold for Cancer Research. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cancer research | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cell culture | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Silica fiber | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Three-dimensional culture system | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Tissueoid | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
MURAKAMI, Shoko
× MURAKAMI, Shoko× MUKAISHO, Ken-ichi× IWASA, Takuya× KAWABE, Masaaki× YOSHIDA, Saori× TANIURA, Naoko× NAKAYAMA, Takahisa× NOI, Masaharu× YAMAMOTO, Gaku× SUGIHARA, Hiroyuki |
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著者別名 |
村上, 翔子
× 村上, 翔子× 向所, 賢一× 吉田, 沙織× 仲山, 貴永× 野井, 将大× 山本, 学× 杉原, 洋行 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: We developed a 3-dimensional (3D) culture system using a high-purity silica fiber scaffold of unwoven sheets called CellbedTM. Methods: We used adherent colon and esophagogastric junction adenocarcinoma cells, tongue squamous cell carcinoma (SqCC) cells, and nonadherent gastric cancer cells. These cells were subjected to staining with various substances and observed by electron microscopy. To evaluate the effects of extracellular matrix in carcinoma tissues, SqCC cells were cultured in Cellbed coated with collagens I, III, and IV. Results: Especially well-differentiated carcinoma cells cultured in this 3D system showed their own unique characteristics: luminal formation in adenocarcinoma cells and cell stratification and keratinization in SqCC cells. Scanning electron microscopy revealed the proliferation of cancer cells with cytoplasm entwined in Cellbed. Intercellular desmosomes in squamous epithelia were detected by transmission electron microscopy of vertical cross sections. SqCC cells cultured in Cellbed coated with collagen IV showed enhanced invasive and proliferative abilities. Conclusion: Because the morphology of cancer cells cultured in this 3D culture system is similar to that in living organisms, we called the system a "tissueoid cell culture system." Coating with collagen IV enables the modification of cell-matrix interactions as well as recapitulation of the in vivo microenvironment. |
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言語 | en | |||||
書誌情報 |
en : Pathobiology : journal of immunopathology, molecular and cellular biology p. 1-11, 発行日 2020-09-23 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Karger | |||||
ISSN | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 1423-0291 | |||||
PMID | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 32966983 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1159/000509133 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | © 2020 S. Karger AG, Basel. | |||||
著者版フラグ | ||||||
出版タイプ | NA | |||||
出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |