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Effects of FTMT Expression by Retinal Pigment Epithelial Cells on Features of Angiogenesis.
http://hdl.handle.net/10422/00012728
http://hdl.handle.net/10422/000127280e5caaef-5c5e-49db-bc33-ddff905e514f
名前 / ファイル | ライセンス | アクション |
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ijms21103635 (4.8 MB)
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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-06-23 | |||||
タイトル | ||||||
タイトル | Effects of FTMT Expression by Retinal Pigment Epithelial Cells on Features of Angiogenesis. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | age-related macular degeneration | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | angiogenesis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | differentiation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | mitochondrial ferritin | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | retinal pigment epithelium | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | vascular endothelial growth factor | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
BUYANDELGER, Undral
× BUYANDELGER, Undral× WALKER, Douglas Gordon× YANAGISAWA, Daijiro× MORIMURA, Toshifumi× TOOYAMA, Ikuo |
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著者別名 |
柳沢, 大治郎
× 柳沢, 大治郎× 守村, 敏史× 遠山, 育夫 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Aberrant angiogenesis is a pathological feature of a number of diseases and arises from the uncoordinated expression of angiogenic factors as response to different cellular stresses. Age-related macular degeneration (AMD), a leading cause of vision loss, can result from pathological angiogenesis. As a mutation in the mitochondrial ferritin (FTMT) gene has been associated with AMD, its possible role in modulating angiogenic factors and angiogenesis was investigated. FTMT is an iron-sequestering protein primarily expressed in metabolically active cells and tissues with high oxygen demand, including retina. In this study, we utilized the human retinal pigment epithelial cell line ARPE-19, both as undifferentiated and differentiated cells. The effects of proinflammatory cytokines, FTMT knockdown, and transient and stable overexpression of FTMT were investigated on expression of pro-angiogenic vascular endothelial growth factor (VEGF) and anti-angiogenic pigment epithelial-derived factor (PEDF). Proinflammatory cytokines induced FTMT and VEGF expression, while NF-κB inhibition significantly reduced FTMT expression. VEGF protein and mRNA expression were significantly increased in FTMT-silenced ARPE-19 cells. Using an in vitro angiogenesis assay with endothelial cells, we showed that conditioned media from FTMT-overexpressing cells had significant antiangiogenic effects. Collectively, our findings indicate that increased levels of FTMT inhibit angiogenesis, possibly by reducing levels of VEGF and increasing PEDF expression. The cellular models developed can be used to investigate if increased FTMT may be protective in angiogenic diseases, such as AMD. | |||||
書誌情報 |
en : International Journal o f Molecular Sciences 巻 21, 号 10, p. 3635, 発行日 2020-05-21 |
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出版者 | ||||||
出版者 | MDPI | |||||
PMID | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 32455741 | |||||
PMCID | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.ncbi.nlm.nih.gov/pmc/articles/pmc7279371/ | |||||
関連名称 | PMC7279371 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.3390/ijms21103635 | |||||
関連名称 | 10.3390/ijms21103635 | |||||
権利 | ||||||
権利情報 | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal Article |